综合生物信息学分析神经胶质瘤中的关键基因及信号通路  

Comprehensive bioinformatic analysis of key genes and signaling pathways in glioma

作　　者：张晓明[1,2] 蒋梦园 汤深凤 牛朝诗 胡珊珊 Xiaoming Zhang;Mengyuan Jiang;Shenfeng Tang;Chaoshi Niu;Shanshan Hu(Department of Neurosurgery,the First Affiliated Hospital of USTC,Division of Life Sciences and Medicine,University of Science and Technology of China,Hefei 230001,China;Anhui Key Laboratory of Brain Function and Diseases,Hefei 230001,China)

机构地区：[1]中国科学技术大学附属第一医院神经外科,安徽合肥230001 [2]脑功能与脑疾病安徽省重点实验室,安徽合肥230001

出　　处：《中国科学技术大学学报》2022年第9期21-33,I0002,共14页JUSTC

基　　金：the National Natural Science Foundation of China(81902525);USTC Research Funds of t he Double First-Class Initiative(YD9100002002)。

摘　　要：鉴定出与患者生存相关的特异性差异表达基因(DEGs)是一种揭示各种癌症(包括神经胶质瘤)治疗方法的新策略。然而,与胶质瘤发生发展相关的关键靶基因仍有待进一步发现。本文对17个GSE数据集进行了生物信息学分析,确定了神经胶质瘤相关的DEGs。我们发现了74个共同DEGs,这些DEGs在胶质瘤中的表达水平较正常组织显著降低,且其与GABA能突触传递、氯离子跨膜转运、谷氨酸分泌、γ-氨基丁酸信号通路有关。γ-氨基丁酸A型受体亚基γ2[GABRG2]被鉴定为蛋白互作网络中的关键基因,在IDH野生型星型胶质瘤中,GABRG2的表达较IDH突变型明显降低,其与胶质瘤患者的预后不良有关。GABRG2可能通过影响GABA受体相关通路促进胶质瘤的发生发展,是胶质瘤诊疗中的潜在生物学标志物。The identification of specific survival-related differentially expressed genes(DEGs)is a method for uncovering therapeutic approaches for various cancers,including glioma.However,the key target genes associated with the occurrence and development of gliomas remain unknown.In this study,we performed bioinformatics analysis on 17 GSE datasets and identified DEGs correlated with glioma.A total of 74 mutual-DEGs with downregulated expression in gliomas compared with that in normal brain tissues were found in 17 datasets.These DEGs were related to GABAergic synaptic transmission,chloride transmembrane transport,glutamate secretion,and gamma-aminobutyric acid signaling pathway.Gamma-aminobutyric acid type A receptor subunit gamma 2(GABRG2)was identified as a hub gene in the protein-protein interaction network.GABRG2 exhibited lower expression in IDH wild-type astrocytoma than that in IDH mutant astrocytoma and indicated poor prognosis in glioma patients.GABRG2 may contribute to the progression of glioma by affecting GABA receptor-related pathways and is a potential biomarker for the diagnosis and treatment of glioma.

关 键 词：胶质瘤 生物信息学分析 GABRG2 生物学标志物 

分 类 号：R739.41[医药卫生—肿瘤]