小鼠淋巴结及胸腺T、B淋巴细胞的鉴别  

IDENTIFICATION OF MOUSE LYMPH NODE AND THYMUS T,B LYMPHOCYTE

作　　者：顾远锡[1] 崔玉芳[1] 徐海超 Gu Yuan Xi;Tsui Yu Fang;Xu Hai Chao(Institute of Radiation Medicine,Academy of Military Medical Science)

机构地区：[1]军事医学科学院放射医学研究所

出　　处：《Zoological Research》1983年第2期191-196,I0005,共7页动物学研究（英文）

摘　　要：本文用细胞化学ACP偶氮染色法和5′N“Gomori”染色法鉴别小鼠淋巴结及胸腺T、B淋巴细胞。测定小鼠上述淋巴组织细胞悬液样品中ACP及5′N活力所得的结果,淋巴结的ACP阳性细胞为67%左右,5′N阳性细胞为31%左右,胸腺的ACP阳性细胞为97%,5′N阳性细胞为10%。上述结果是相互符合的,也与目前已报导的小鼠及人的淋巴结和胸腺T、B淋巴细胞百分数基本符合。文中就ACP偶氮染色法与非特异性酯酶染色法进行了比较,证明在小鼠上述淋巴组织细胞悬液中ACP阳性淋巴细胞基本上代表T淋巴细胞。还就5′N“Gomori”法染色过程中,基质液中铅离子浓度对5′N活力的影响,ACP活力抑制剂的使用和机理以及所得出的小鼠淋巴结及胸腺中T,B淋巴细胞百分数的可靠性进行了初步讨论。在深入研究机体细胞免疫和体液免疫以及二者的相互关系所进行免疫功能的测定方面,应用免疫学方法的报导较多,而有关细胞化学的应用则甚少,如非特异性酯酶染色法鉴别T淋巴细胞的资料(Mueller,1975;1976;Ranki,1976),以及鉴别B淋巴细胞的零星记载。The azo dye method for acid phosphatase and "Gomori" method for 5’ Nucleotidase were used to identify the T,and B lymphocytes of mouse lymph node and thymus cytochemically.By employing these methods,the estimation of ACP and 5’N activities of parallel samples from the lymph node and thymus cell suspensions were carried out in order to verify the ACP and 5’N positive lymphocyte percentages obtained previously.The ACP and 5’N positive lymphocytes thus estimated for lymph node were found to be 67% and 31%,whereas those for the thymus were around 97% and 10% respectively.Above mentioned results were not only well fitted with each other but also in good accordance with those already published.In addition,from the following poins = the enzyme reaction products,the relationship between T-cell acid phosphatase pattern and T-cell esterasc pattern in 6 normal individuals,the number of T-cell acid phosphatase and T-cell es-terase pattern in resette preparations and the ultrastructural cytochemistry,the azo dye method for acid phosphatase was compared with the nonspecific esterase staining method for the T lymphocyte identification.It was proved that these two methods were of the same value in the T lymphocyte identification of mouse lymph node and thymus cell suspensions.Furthermore,the effect of different lead concentrations in the incubating medium on 5’N activity,the use of ACP inhibitor and its possible mechanism and the reliability of T,B lymphocyte percentages of mouse lymph node and thymus were also briefly discussed.

关 键 词：机体细胞免疫 应用免疫学 非特异性酯酶 基质液 B淋巴细胞 细胞化学 T淋巴细胞 淋巴结 

分 类 号：R73[医药卫生—肿瘤]