SFRP1及Dickkopf相关蛋白1在慢性牙周炎患者牙龈组织中的表达及意义  

Expression and significance of SFRP1 and DKK1 in gingival tissue of patients with chronic periodontitis

作　　者：陈欣[1] 尼加提·吐尔逊[1] 热依拉·库尔班 CHEN Xin;NIJIATI·Tu-er-xun;REYILA·Ku-er-ban(Department of Stomatology,the Second Affiliated Hospital of Xinjiang Medical University,Urumqi 830001,Xinjiang,China)

机构地区：[1]新疆医科大学第二附属医院口腔科,新疆830001

出　　处：《川北医学院学报》2022年第9期1106-1109,1119,共5页Journal of North Sichuan Medical College

基　　金：新疆维吾尔自治区自然科学基金(2019D01C226)。

摘　　要：目的:通过检测分泌型卷曲相关蛋白(SFRP1)及Dickkopf相关蛋白1(DKK1)在慢性牙周炎(CP)患者牙龈组织中的表达改变,探讨SFRP1和DKK1联合检测对慢性牙周炎感染的临床意义。方法:选取CP患者30例(CP组),根据牙周炎病变程度分为轻度CP组(n=13)和重度CP组(n=17);另以15名健康志愿者为对照组,采用免疫组织化学技术检测SFRP1和DKK1在牙龈组织中的表达,染色强度运用双评分法评价。采用牙龈组织蛋白印迹法检测SFRP1和DKK1的蛋白表达水平。结果:免疫组织化学实验检测结果显示,轻度CP组的SFRP1和DKK1阳性积分值高于正常组,重度CP组高于轻度CP组,差异均有统计学意义(P<0.05)。Western blot实验检测结果显示,轻度CP组的SFRP1和DKK1蛋白表达水平高于正常组,重度CP组高于轻度CP组,差异有统计学意义(P<0.05)。结论:SFRP1与DKK1在CP患者牙龈组织中的表达升高,且两者的表达水平可能与病情严重程度有关。Objective:To investigate the clinical significance of secreted crimp related protein(SFRP1) and DKKOPF-related protein 1(DKK1) combined detection in the gingival tissue of chronic periodontitis(CP) by detecting the expression changes of secreted curl associated protein(SFRP1) and DicKKOPF-related protein 1(DKK1) in patients with CP.Methods:30 CP patients(CP group) were selected and divided into moderate CP group(n=13) and severe CP group(n=17) according to the severity of periodontitis, and 15 healthy controls as the healthy group.Expressions of SFRP1 and DKK1 in gingival tissue were detected by immunohistochemistry, and the staining intensity was evaluated by double score method.Protein expression levels of SFRP1 and DKK1 were detected by western blot in gingival tissue.Results:The immunohistochemical experiment results showed that the positive scores of SFRP1 and DKK1 in the mild CP group were higher than those in the normal group, and the positive scores of SFRP1 and DKK1 in the severe CP group were higher than those in the mild CP group, and the differences between groups were statistically significant(P<0.05).The WB results showed that the expression levels of SFRP1 and DKK1 proteins in the mild CP group were higher than those in the normal group, and the expression levels of SFRP1 and DKK1 proteins in the severe CP group were higher than those in the mild CP group, and the differences between groups were statistically significant(P<0.05).Conclusion:The expression of SFRP1 and DKK1 is elevated in the gingival tissue of CP patients and the expression levels of both may be related to the severity of the disease.

关 键 词：慢性牙周炎 分泌型卷曲蛋白1 Dickkopf相关蛋白1 

分 类 号：R781.4[医药卫生—口腔医学]