In Situ Saturating Mutagenesis Screening Identifies a Functional Genomic Locus that Regulates Ucp1 Expression  

作　　者：Yan Qiu Xiaojian Liu Yingmin Sun Shuang Li Yuda Wei Cheng Tian Qiurong Ding 

机构地区：[1]CAS Key Laboratory of Nutrition,Metabolism and Food Safety,Shanghai Institute of Nutrition and Health,University of Chinese Academy of Sciences,Chinese Academy of Sciences,Shanghai 200031,People’s Republic of China [2]Institute for Stem Cell and Regeneration,Chinese Academy of Sciences,Beijing 100101,People’s Republic of China

出　　处：《Phenomics》2021年第1期15-21,共7页表型组学（英文）

基　　金：supported by grants from the National Key R&D Program of China(2017YFA0102800,2017YFA0103700);the Strategic Priority Research Program of the Chinese Academy of Sciences(XDA16030402);the National Natural Science Foundation of China(31670829,31971063).

摘　　要：A better understanding of the molecular mechanisms that control the UCP1 expression in brown and beige adipocytes is essential for us to modulate adipose cell fate and promote thermogenesis,which may provide a therapeutic view for the treatment of obesity and obesity-related diseases.To systematically identify cis-element(s)that transcriptionally regulates Ucp1,we here took advantage of the high-throughput CRIPSR-Cas9 screening system,and performed an in situ saturating mutagenesis screen,by using a customized sgRNA library targeting the~20 kb genomic region near Ucp1.Through the screening,we have identified several genomic loci that may contain key regulatory element for Ucp1 expression in cultured brown and white adipocytes in vitro,and in inguinal white adipose tissue in vivo.Our study highlights a broadly useful approach for studying cis-regulatory elements in a high-throughput manner.

关 键 词：In situ saturating mutagenesis screen Brown and beige adipocytes UCP1 sgRNA-9768 

分 类 号：R73[医药卫生—肿瘤]