基于癌症基因组图谱数据库探索miR-217在胃癌中的表达及其预后价值  

Expression and prognostic significance of miR-217 in patients with gastric cancer:the Cancer Genome Atlas Database based study

作　　者：郑侠 邵杰[1] 谷雨[1] 潘宇[1] ZHENG Xia;SHAO Jie;GU Yu;PAN Yu(Department of Oncology,Jiangsu Province Hospital of Chinese Medicine,Affiliated Hospital of Nanjing University of Chinese Medicine,Nanjing 210029,China)

机构地区：[1]江苏省中医院南京中医药大学附属医院肿瘤科,南京210029

出　　处：《临床肿瘤学杂志》2022年第9期788-793,共6页Chinese Clinical Oncology

摘　　要：目的评估miR-217在胃癌组织中的表达及其预后价值。方法利用R语言统计学软件下载癌症基因组图谱数据库(TCGA)胃癌数据集中患者的临床病理学资料、生存数据和miRNA测序数据,比较miR-217在肿瘤组织与癌旁组织间的表达差异,受试者操作特征曲线(ROC)评估miR-217对胃癌的诊断价值;Kaplan-Meier生存分析比较不同miR-217表达水平的胃癌患者无进展时间(PFI)和总生存期(OS);利用单因素和多因素Cox回归分析评估影响PFI和OS的预后因子;利用基因本体论(GO)和京都基因与基因组百科全书(KEGG)对miR-217潜在靶基因进行功能和信号通路富集分析。结果446例胃癌患者纳入分析,Wilcoxon秩和检验结果显示,miR-217显著高表达于胃癌组织(5.10 vs.3.74,P<0.01)。经ROC曲线评估发现,miR-217具有良好的诊断价值(AUC=0.795,95%CI:0.728~0.863)。Kaplan-Meier结果显示,与低表达组比较,miR-217高表达的患者OS更短(56.2个月vs.22.3个月,P=0.005),但两组患者PFI之间的差异无统计学意义(55.9个月vs.26.0个月,P=0.099)。多因素Cox回归分析结果显示,miR-217是影响患者OS的独立预后因子之一(HR=1.386,95%CI:1.003~1.914,P=0.048),但miR-217的表达与患者PFI无明显相关性(P>0.05)。GO/KEGG富集分析结果表明,miR-217潜在的靶基因主要富集于前列腺癌、膀胱癌、黑色素瘤、子宫内膜癌、EGFR酪氨酸激酶抑制剂抵抗、细胞间蛋白转运调节、蛋白入核调节、神经分支形态、成长发育负向调节及核-浆转运调控等病理过程。结论miR-217显著高表达于胃癌组织,其表达水平可用于预测胃癌患者的死亡风险;miR-217的生物学功能可能与泌尿生殖系统恶性肿瘤以及蛋白质转运等相关。Objective To determine the expression and prognostic significance of miR-217 in patients with gastric cancer.Methods We searched and downloaded miRNA-seq data in gastric cancer tissues,patients’clinicopathological information and survival data from The Cancer Genome Atlas(TCGA)database.Initially,the expression of miR-217 between tumor tissues and adjacent tissues were compared.The diagnostic value of miR-217 was assessed by receiver operating characteristic(ROC)curve.Next,Kaplan-Meier analysis was utilized to compare overall survival(OS)and progression free interval(PFI)between patients with high and low miR-217 levels.The underlying prognostic factors were assessed by univariate and multivariate Cox regression analysis.Finally,we used Gene Ontoloty(GO)and Kyoto Encyclopedia of Genes and Genomes(KEGG)enrichment analysis to assess the function and pathways of the candidate target genes of miR-217.Results There were 446 gastric cancer patients included in presents study.After a Wilcoxon rank sum test,it uncovered that miR-217 expressed higher in tumor tissues than in adjacent tissues(5.10 vs.3.74,P<0.01).Additionally,ROC curve analysis results demonstrated that miR-217 performed an outstanding diagnostic value in gastric cancer(AUC=0.795,95%CI:0.728-0.863).Kaplan-Meier analysis revealed that patients with an increased miR-217 level performed a poor OS(56.2 months vs.22.3 months,P=0.005)instead of PFI(55.9 months vs.26.0 months,P=0.099).According to the results of multivariate analysis,it demonstrated that miR-217 was one of the independent prognostic factors for predicting OS(HR=1.386,95%CI 1.003-1.914,P=0.048)rather than PFI.After GO/KEGG enrichment analysis to the potential targeted genes,it revealed that these genes enriched to prostates cancer,bladder cancer,melanoma,endometrial cancer,EGFR tyrosine kinase inhibitor resistance,regulation of intracellular protein transport,branching morphogenesis of a nerve,negative regulation of developmental growth,regulation of nucleocytoplasmic transport.Conclusion miR-217

关 键 词：胃癌 miR-217 预后因子 癌症基因组图谱数据库 

分 类 号：R735.2[医药卫生—肿瘤]