Rottlerin plays an antiviral role at early and late steps of Zika virus infection  

作　　者：Shili Zhou Quanshi Lin Changbai Huang Xiaotong Luo Xu Tian Chao Liu Ping Zhang 

机构地区：[1]Key Laboratory of Tropical Diseases Control(Sun Yat-sen University),Ministry of Education,Guangzhou,510080,China [2]Medical Research Center,Guangdong Second Provincial General Hospital,Guangzhou,510317,China [3]Department of Immunology,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,510080,China [4]Department of Microbiology,Zhongshan School of Medicine,Sun Yat-sen University,Guangzhou,510080,China

出　　处：《Virologica Sinica》2022年第5期685-694,共10页中国病毒学（英文版）

基　　金：supported by Guangzhou Municipal Science and Technology Program (202206010114);National Natural Science Foundation of China (31970887, 92169110);Doctoral workstation foundation of Guangdong Second Provincial General hospital (2020BSGZ036);Natural Science Foundation of Guangdong Province(2021A1515011491, 2022A1515010451)

摘　　要：Infection of Zika virus(ZIKV)may cause microcephaly and other neurological disorders,while no vaccines and drugs are available.Our study revealed that rottlerin confers a broad antiviral activity against several enveloped viruses,including ZIKV,vesicular stomatitis virus,and herpes simplex virus,but not against two naked viruses(enterovirus 71 and encephalomyocarditis virus).Rottlerin does not have a direct virucidal effect on the virions,and its antiviral effect is independent of its regulation on PKCδor ATP.Both pretreatment and post-treatment of rottlerin effectively reduce the viral replication of ZIKV.The pretreatment of rottlerin disturbs the endocytosis of enveloped viruses,while the post-treatment of rottlerin acts at a late stage through disturbing the maturation of ZIKV.Importantly,administration of rottlerin in neonatal mice significantly decreased the ZIKV replication in vivo,and alleviated the neurological symptoms caused by ZIKV.Our work suggests that rottlerin exerts an antiviral activity at two distinct steps of viral infection,and can be potentially developed as a prophylactic and therapeutic agent.

关 键 词：Zika virus(ZIKV) ROTTLERIN Enveloped virus REPLICATION 

分 类 号：R373[医药卫生—病原生物学]