低共熔溶剂协同酸性二氧六环预处理杨木及其酶水解特性的探究  

Synergistic optimization of deep eutectic solvent and acidic dioxane pretreatment on the enhanced enzymatic hydrolysis of poplar

作　　者：徐春燕 熊凤 陈德明 单玉蓉 张林[1,2,3] 王挥 XU Chun-yan;XIONG Feng;CHEN De-ming;SHAN Yu-rong;ZHANG Lin;WANG Hui(College of Materials Science and Engineering,Central South University of Forestry and Technology,Changsha 410000,China;Ministry of Forestry Bioethanol Research Center,Changsha 410000,China;Hunan International Joint Laboratory of Woody Biomass Conversion,Changsha 410000,China)

机构地区：[1]中南林业科技大学材料科学与工程学院,湖南长沙410000 [2]国家林业局生物乙醇研究中心,湖南长沙410000 [3]湖南省木质资源定向转化国际联合实验室,湖南长沙410000

出　　处：《应用化工》2022年第9期2558-2564,共7页Applied Chemical Industry

基　　金：国家重点研发计划资助项目(2018YFB1501401);湖南省自然科学基金项目(2019JJ40539)。

摘　　要：以杨木为原料,采用低共熔溶剂(DES)协同酸性二氧六环进行预处理,研究盐酸浓度、预处理时间和预处理温度对杨木酶水解特性的影响。结果表明,当盐酸质量分数为1%,反应温度为110℃,时间为70 min时,酶水解得率为69.75%,纤维素保留率为73.66%、半纤维素和木质素脱除率分别为72.61%和48.71%。协同预处理在保留纤维素的同时,可显著提高杨木中半纤维素和木质素的脱除率。同时,预处理能够大大提高杨木的结晶度,破坏杨木的致密结构,影响其热稳定性,进而有效提高纤维素酶水解得率。Using poplar as raw material,the pretreatment of deep eutectic solvent(DES)with acidic dioxane has been performed to promote the enzymatic digestibility of poplar.The influence of hydrochloric acid concentration,pretreatment time and pretreatment temperature on poplar enzymatic hydrolysis characteristic was investigated.The optimal condition was 110℃for 70 min,containing 1.0%HCl,and 69.75%was converted after enzymatic hydrolysis.The cellulose recovery was 73.66%,hemicellulose removal and lignin removal rate were 72.61%and 48.71%,respectively.The results presented the applied method has strongly selectivity towards removal of lignin and hemicellulose while retaining cellulose.Furthermore,pretreatment can greatly accelerate the crystallinity of poplar,destroy its structure and affect thermal stability of poplar.Thus,the enzymatic hydrolysis yield was effectively improved.

关 键 词：木质纤维素 协同预处理 酶水解 葡萄糖得率 

分 类 号：TQ353.4[化学工程] TQ353.2[动力工程及工程热物理—生物能] TK6