Highly efficient enzymolysis and fermentation of corn stalk into L-lactic acid by enzyme-bacteria friendly ionic liquid pretreatment  

作　　者：Yongqing Yang Yuehai Wang Xingmei Lu Xu Zheng Dongxia Yan Jiayu Xin Ibrahim El-Tantawy El-Sayed Ying Kang Jiming Yang 

机构地区：[1]Beijing Key Laboratory of Ionic Liquids Clean Process,Key Laboratory of Green Process Engineering,Institute of Process Engineering,Chinese Academy of Sciences,Beijing,100190,China [2]School of Chemistry and Chemical Engineering,University of Chinese Academy of Sciences,Beijing,100049,China [3]Department of Chemistry,Faculty of Science,Menoufia University,Shebin El-Kom,Menoufia,32511,Egypt [4]Innovation Academy for Green Manufacture,Chinese Academy of Sciences,Beijing,100190,China [5]Dalian National Laboratory for Clean Energy,Dalian Institute of Chemical Physics,Chinese Academy of Sciences,Dalian,116023,China

出　　处：《Green Chemical Engineering》2022年第4期321-327,共7页绿色化学工程（英文）

基　　金：funded by the National Key R&D Program of China(Grant numbers:No.2021YFE0190800,No.2018YFB1501600);the Strategic Priority Research Program of Chinese Academy of Science(No.XDA21060300,No.XDA24030504);National Natural Science Foundation of China(No.21878292,No.21878314);K.C.Wong Education Foundation(No.GJTD-2018-04);the Joint fund of Innovation Academy for Green Manufacture(No.IAGM2020C12,No.IAGM2020C21);Chinese Academy of Sciences President's International Fellowship Initiative(Grant No.122111WGZJTPYJY20180050).

摘　　要：Ionic liquids(ILs)have been widely used in the pretreatment of biomass.However,the effects of residual ILs on the enzymolysis and fermentation of biomass are still unknown.Therefore,a large quantity of water-washing is usually followed after biomass pretreatment to eliminate the inhibition of residual ILs on subsequent hydrolysis and fermentation steps.In this work,the effect of choline glycine([Ch][Gly])concentration on the activity of cellulase and Bacillus sp.strain P38 was systematically investigated to explore the impacts of residual ILs on enzymolysis and fermentation.The results confirmed that the activities of them were almost not inhibited in low concentrations(less than 0.5 wt%)of[Ch][Gly].Under optimal pretreatment conditions,the maximum cellulose digestibility was 99.23%.Enzymatic hydrolysate was suitable for L-lactic acid fermentation without appreciable inhibition,and the highest sugar-acid conversion rate of 96.33%was obtained by simplified detoxification.This work provides an economic route to produce fermentable sugar and L-lactic acid,which shows an industrial application prospect in lignocellulosic biorefinery.

关 键 词：Corn stalk BIOCOMPATIBILITY Choline glycine Enzymatic hydrolysis L-lactic acid fermentation 

分 类 号：TQ921.3[轻工技术与工程—发酵工程]