Dietary cholesterol promotes growth and ecdysone signalling pathway by modulating cholesterol transport in swimming crabs(Portunus trituberculatus)  

作　　者：Tingting Zhu Qicun Zhou Zheng Yang Yingying Zhang Jiaxiang Luo Xiangsheng Zhang Yuedong Shen Lefei Jiao Douglas R.Tocher Min Jin 

机构地区：[1]Laboratory of Fish and Shellfish Nutrition,School of Marine Sciences,Ningbo University,315211 Ningbo,China [2]Guangdong Provincial Key Laboratory of Marine Biotechnology,Institute of Marine Sciences,Shantou University,515063 Shantou,China

出　　处：《Animal Nutrition》2022年第3期249-260,共12页动物营养（英文版）

基　　金：the National Natural Science Foundation of China(32072987);China Agriculture Research System-48(Supported by China Agriculture Research System of MOF and MARA);National Key R&D Program of China(2018YFD0900400);K.C.Wong Magna Fund in Ningbo University.

摘　　要：Cholesterol,as an indispensable nutrient,regulates molting and growth in crustacean.As crustaceans are unable to biosynthesize cholesterol de novo,it is central to understand how dietary cholesterol affects molting in crustaceans.An 8-week feeding trial was conducted to evaluate the effects of dietary cholesterol level(0.12%,0.43%,0.79%,1.00%,1.30%and 2.50%)on growth,cholesterol metabolism and expression of genes related to lipid and ecdysone metabolism in female swimming crabs(Portunus trituberculatus).A total of 192 crabs(1.41±0.05 g)were randomly distributed into 192 aquaria.Each treatment had 4 replicates with each replicate containing 8 crabs.Crabs fed the 1.00%cholesterol diet showed best growth performance,and thus based on percent weight gain,the optimal dietary cholesterol requirement was calculated at 1.01%.Tissue cholesterol concentrations were positively correlated with dietary cholesterol level.The contents of functional fatty acids in hepatopancreas significantly increased as dietary cholesterol increased from 0.12%to 2.50%(P<0.05).The expression levels of genes related to lipogenesis pathway,lipid catabolism and fatty acid oxidation were significantly downregulated with increased dietary cholesterol level(P<0.05).The highest expression levels of cholesterol transport genes,low-density lipoprotein receptor(ldlr)and low-density lipoprotein receptorrelated protein 2(lrp2)occurred in crabs fed the 1.30%cholesterol diet.Moreover,hormones related to molting such as crustacean hyperglycemic hormone(CHH),methyl farnesoate(MF),molt-inhibiting hormone(MIH),and ecdysone in hemolymph were significantly influenced by dietary cholesterol level(P<0.05).The highest expression levels of ecdysone receptor(ecr)and chitinase 1(chi1)in eyestalk and hepatopancreas were found in crabs fed the diet containing 1.00%cholesterol(P<0.05).In conclusion,the optimal dietary level was beneficial to functional fatty acid accumulation,regulated lipid metabolism,promoted the ecdysone signalling pathway by improving the cholesterol

关 键 词：Portunus trituberculatus CHOLESTEROL Lipid metabolism Ecdysone signalling pathway 

分 类 号：S968.252[农业科学—水产养殖]