Dietary supplementation of sulfur amino acids improves intestinal immunity to Eimeria in broilers treated with anti-interleukin-10 antibody  

作　　者：Zhouzheng Ren Jiakun Yan Rose Whelan Xujie Liao Daniel E.Bütz Maria K.Arendt Mark E.Cook Xiaojun Yang Thomas D.Crenshaw 

机构地区：[1]College of Animal Science and Technology,Northwest A&F University,Yangling,Shaanxi,China [2]Department of Animal Sciences,University of Wisconsin,Madison,WI,USA [3]Evonik Operations GmbH,Hanau-Wolfgang,Germany

出　　处：《Animal Nutrition》2022年第3期382-389,共8页动物营养（英文版）

基　　金：Innovative Research Team of Animal Nutrition&Healthy Feeding at the Northwest A&F University for their help in data analysis and manuscript preparation.This research was supported in part by the National Natural Science Foundation of China(31902175);Young Science and Technology Star Project of Shaanxi Province(2021KJXX-97);Basic Scientific Research Business Expenses at the Northwest A&F University(College of Animal Science and Z.Ren,J.Yan,R.Whelan et al.Animal Nutrition 10(2022)382e389388 Technology,DK202103);Evonik Operations GmbH(Hanau-Wolfgang,Germany).

摘　　要：Oral antibody to interleukin-10(anti-IL-10)enhances the intestinal immune defense against Eimeria.The sulfur amino acids methionine and cysteine(MtC)play essential roles in inducing and maintaining protective immune responses during intestinal infections.Hence,increased dietary MtC may support the anti-IL-10-induced intestinal immunity to Eimeria.Broilers(n=640)were arranged in a 2×2×2 factorial design with 2 levels of each of the 3 main factors:dietary standardized ileal digestible(SID)MtC levels(0.6%or 0.8%),dietary anti-IL-10 supplementation(with or without),and coccidiosis challenge(control or challenge).Briefly,the broilers were supplied with either 0.6%or 0.8%SID MtC,each with or without anti-IL-10(300 mg/kg),from d 10 to 21.On d 14,broilers from each diet were gavaged with either PBS or Eimeria.The resulting Eimeria infection induced fecal oocyst shedding and intestinal lesions.Broilers fed 0.8%SID MtC(main effects,P≤0.05)had decreased feed-to-gain ratio,increased duodenum and cecum luminal anti-Eimeria IgA titers,and decreased fecal oocyst counts,when compared to 0.6%SID MtC.The supplementation of anti-IL-10(main effects,P≤0.05)increased cecum luminal total IgA concentration and decreased cecum lesions.Interactions(P≤0.05)were detected for growth performance and cecum luminal IFN-γ.Briefly,the highest body weight gain and feed intake were reached in PBS-gavaged broilers fed 0.8%SID MtC with no anti-IL-10 and in Eimeria-challenged broilers fed 0.8%SID MtC with anti-IL-10.In Eimeria-infected broilers,anti-IL-10 increased intestinal luminal IFN-γ and body weight gain only at 0.8%SID MtC.Collectively,anti-IL-10 increased intestinal luminal IFN-γ levels,decreased cecum lesions and restored growth only when fed with adequate amounts of sulfur amino acids.Our findings underscore the importance of providing sufficient essential nutrients to support the anti-IL-10 induced immunity against coccidiosis.

关 键 词：Chicken COCCIDIOSIS Egg yolk antibody INTERLEUKIN-10 Sulfur amino acid 

分 类 号：S831.5[农业科学—畜牧学]