Enhancement and mapping of tolerance to salt stress and 5-fluorocytosine in synthetic yeast strains via SCRaMbLE  

作　　者：Jianping Kang Jieyi Li Zhou Guo Sijie Zhou Shuxin Su Wenhai Xiao Yi Wu Yingjin Yuan 

机构地区：[1]Frontier Science Center for Synthetic Biology(Ministry of Education),Tianjin University,Tianjin,300072,China [2]Key Laboratory of Systems Bioengineering(Ministry of Education),School of Chemical Engineering and Technology,Tianjin University,Tianjin,300072,China

出　　处：《Synthetic and Systems Biotechnology》2022年第3期869-877,共9页合成和系统生物技术（英文）

基　　金：supported by the National Key R&D Program of China(2021YFC2102500);National Natural Science Foundation of China(31971351).

摘　　要：Varied environmental stress can affect cell growth and activity of the cellular catalyst.Traditional path of adaptive evolution generally takes a long time to achieve a tolerance phenotype,meanwhile,it is a challenge to dissect the underlying genetic mechanism.Here,using SCRaMbLE,a genome scale tool to generate random structural variations,a total of 222 evolved yeast strains with enhanced environmental tolerances were obtained in haploid or diploid yeasts containing six synthetic chromosomes.Whole genome sequencing of the evolved strains revealed that these strains generated different structural variants.Notably,by phenotypic-genotypic analysis of the SCRaMbLEd strains,we find that a deletion of gene YFR009W(GCN20)can improve salt tolerance of Saccharomyces cerevisiae,and a deletion of gene YER056C can improve 5-flucytosine tolerance of Saccharomyces cerevisiae.This study shows applications of SCRaMbLE to accelerate phenotypic evolution for varied environmental stress and to explore relationships between structural variations and evolved phenotypes.

关 键 词：SCRAMBLE Saccharomyces cerevisiae Structural variation GCN20 TOLERANCE 

分 类 号：Q78[生物学—分子生物学]