Whole lifecycle observation of single‐spore germinated Streptomyces using a nanogap‐stabilized microfluidic chip  

作　　者：Dongwei Chen Mengyue Nie Wei Tang Yuwei Zhang Jian Wang Ying Lan Yihua Chen Wenbin Du 

机构地区：[1]State Key Laboratory of Microbial Resources,Institute of Microbiology,Chinese Academy of Sciences,Beijing,China [2]College of Life Sciences,University of the Chinese Academy of Sciences,Beijing,China [3]Savaid Medical School,University of the Chinese Academy of Sciences,Beijing,China

出　　处：《mLife》2022年第3期341-349,共9页微生物（英文）

基　　金：We would like to thank Dr.Liang Ma from Dawei Biotechnologies(Beijing,China)for his insightful suggestion.This study was supported by the National Key Research&Development Program of China(2021YFC2301000,2021YFA0717000,and 2021YFC2103300);the National Natural Science Foundation of China(31970091,91951103,and 21822408).

摘　　要：Streptomyces is a model bacterium to study multicellular differentiation and the major reservoir for antibiotics discovery.However,the cellular‐level lifecycle of Streptomyces has not been well studied due to its complexity and lack of research tools that can mimic their natural conditions.In this study,we developed a simple microfluidic chip for the cultivation and observation of the entire lifecycle of Streptomyces development from the single‐cell perspective.The chip consists of channels for loading samples and supplying nutrients,microwell arrays for the seeding and growth of single spores,and air chambers beside the microwells that facilitate the development of aerial hyphae and spores.A unique feature of this chip is that each microwell is surrounded by a 1.5µm nanogap connected to an air chamber,which provides a stabilized water–air interface.We used this chip to observe the lifecycle development of Streptomyces coelicolor and Streptomyces griseus germinated from single spores,which revealed differentiation of aerial hyphae with progeny spores at micron‐scale water–air interfaces and air chambers.Finally,we demonstrated the applicability of this chip in phenotypic assays by showing that the microbial hormone A‐Factor is involved in the regulatory pathways of aerial hyphae and spore formation.The microfluidic chip could become a robust tool for studying multicellular differentiation,single‐spore heterogeneity,and secondary metabolism of single‐spore germinated Streptomyces.

关 键 词：microfluidics multicellular differentiation single cell analysis single‐spore germination STREPTOMYCES 

分 类 号：Q93[生物学—微生物学]