H101对胃癌细胞增殖、迁移、凋亡的调控作用及其相关机制研究  

Effects of H101 on proliferation,migration,and apoptosis of gastric cancer cells and underlying mechanisms

作　　者：邵苏 徐鹏飞 占小平 Su Shao;Peng-Fei Xu;Xiao-Ping Zhan(Zhejiang Chinese Medical University(Department of General Surgery of Chun An First People’s Hospital),Hangzhou 310053,Zhejiang Province,China;Department of General Surgery,Chun An First People’s Hospital,Hangzhou 311700,Zhejiang Province,China)

机构地区：[1]浙江中医药大学(淳安县第一人民医院)普外科,浙江省杭州市310053 [2]淳安县第一人民医院普外科,浙江省杭州市311700

出　　处：《世界华人消化杂志》2022年第18期803-809,共7页World Chinese Journal of Digestology

基　　金：杭州市医药卫生科技项目,No.B20210327.

摘　　要：背景溶瘤病毒作为治疗癌症的新手段,具有疗效快,靶向性强,无细胞毒性等特点,H101作为商品化上市的溶瘤病毒表现出了稳定的抗癌特效,为中晚期胃癌患者的腹膜转移治疗提供了可能.目的探讨H101对胃癌细胞增殖、迁移、凋亡的调控作用及其相关机制研究.方法将BGC-823和SGC-7901两种胃癌细胞系培养在6孔板中,分别加入感染复数(multiplicity of infection,MOI)值为1、5、10、15的H101,作用48 h后,用MTS法检测细胞的存活率,免疫印迹法(western blot,WB)检测腺病毒早期区1(adenovirus early region 1,E1A)的表达.选取SGC-7901细胞,用划痕法检测各组细胞48 h的迁移率,Hoechst33342染液,观察H101作用48 h后凋亡小体的形成情况.Western blot检测细胞凋亡相关蛋白的表达.结果WB结果显示,H101中的EIA表达量比对照组更高,差异具有统计学意义(P<0.05).MTS结果显示与MOI值为1时相比,MOI值为15的时候,H101在体外可以明显杀伤BGC-823和SGC-79012种胃癌肿瘤细胞(P<0.05).在增殖实验中发现相对于BGC-823细胞,SGC-7901细胞对H101的敏感性更差,因此选取敏感性相对较差的SGC-7901细胞作为研究重点.48 h后,与对照组和MOI=1组的迁移距离相比,MOI=5组,MOI=10组和MOI=15组的迁移距离明显更低,(P均<0.05).与MOI=1组相比,MOI=5、MOI=10和MOI=15组的凋亡小体数量更多(P均<0.05),与对照组和MOI=1组相比,MOI=5、MOI=5和MOI=10组的B淋巴细胞瘤-2蛋白(B-cell lymphoma-2,Bcl-2)表达量较低(P均<0.05),而凋亡蛋白(BCL2-associated X,Bax)、半胱氨酸蛋白酶-3(cysteinyl aspartate specific proteinase-3,Caspase-3)表达量均更高(P均<0.05).结论H101能够抑制胃癌细胞的增殖、迁移,提高细胞凋亡相关蛋白的表达量,加速细胞的凋亡.BACKGROUND As a new means of cancer treatment,oncolytic virus has the characteristics of rapid efficacy,strong targeting,and no cytotoxicity.H101,as a commercially marketed oncolytic virus,has shown stable anticancer effect,which provides a possibility for the treatment of peritoneal metastasis in patients with advanced gastric cancer.AIM To investigate the effects of H101 on proliferation,migration,and apoptosis of gastric cancer cells and the underlying mechanisms.METHODS The gastric cancer cell lines BGC-823 and SGC-7901were cultured in 6-well plates,and H101 with multiplicity of infection(MOI)values of 1,5,10,and 15 were added,respectively.After treatment for 48 h,MTS method was used to detect the cell survival rate,and Western blot was used to detect the expression of E1A.SGC-7901 cells were then selected and cell mobility was detected by the scratch test.Hoechst33342 dye was used to observe the formation of apoptotic bodies after treatment with H101 for 48 h.The expression of apoptosis-related proteins was detected by Western blot.RESULTS Western blot analysis showed that EIA expression in cells treated with H101 was significantly higher than that in the control group(P<0.05).MTS assay showed that H101 with an MOI value of 15 could more significantly kill BGC823 and SGC-79012 cells in vitro than in that with an MOI value of 1(P<0.05).After 48 h,the migration distance of cells in the MOI=5,MOI=10,and MOI=15 groups was significantly lower than that of the control group and MOI=1 group(P<0.05).Compared with the MOI=1 group,the MOI=5,MOI=10,and MOI=15 groups had significantly more apoptotic bodies(P<0.05),and the expression levels of Bcl-2,Bax,and Caspase were significantly higher in the MOI=5,MOI=5,and MOI=10 groups than in the control group and MOI=1 group(P<0.05).

关 键 词：H101 胃癌 增殖 迁移 凋亡 

分 类 号：R735.2[医药卫生—肿瘤]