Profiles of interferon-gamma and interleukin-2 in patients after allogeneic hematopoietic stem cell transplantation  

作　　者：Malwina Rybicka-Ramos Mirosław Markiewicz Aleksandra Suszka-Świtek Ryszard Wiaderkiewicz Sylwia Mizia Monika Dzierżak-Mietła Krzysztof Białas 

机构地区：[1]Department of Hematology,Specialist Hospital No.1 in Bytom,Bytom 41-902,Poland [2]Department of Hematology,University of Rzeszow,College of Medical Sciences,Institute of Medical Sciences,Rzeszów 35-315,Poland [3]Department of Histology and Embryology,School of Medicine in Katowice,Medical University of Silesia,Katowice 40-752,Poland [4]Department of Population Health,Division of Epidemiology and Health Education,Wroclaw Medical University,Wrocław 51-618,Poland [5]Department of Bone Marrow Transplantation and Hematooncology,Maria Sklodowska-Curie National Research Institute of Oncology,Gliwice 44-102,Poland [6]Department of Hematology and Bone Marrow Transplantation,SPSK-M Hospital,Katowice 40-027,Poland

出　　处：《World Journal of Biological Chemistry》2022年第4期72-82,共11页世界生物化学杂志（英文版）（电子版）

摘　　要：BACKGROUND Allogeneic hematopoietic stem cell transplantation(allo-HSCT)may be related to the occurrence of complications,including graft-versus-host disease(GvHD)and infections.The pathogenesis of acute GvHD is connected with T lymphocytes,which identify alloantigens on host's antigen-presenting cells,activate production of interferon-gamma(IFN-gamma)and interleukin-2(IL-2),and act on the immune effector cells and damage tissues and organs.AIM The aim of the study was to investigate and distinguish serum concentration profiles of IFN-gamma and IL-2 within a 30-d period after allo-HSCT.METHODS We enrolled 62 patients,i.e.,30(48%)male and 32(52%)female subjects[median age 49.5(19-68)years],after allo-HSCT from siblings(n=12)or unrelated donors(n=50)due to acute myeloid leukemia with myeloablative conditioning(n=26;42%)and with non-myeloablative conditioning(n=36;58%).All patients were given standard immunosuppressive therapy with cyclosporin-A and methotrexate and pre-transplant antithymocyte globulin in the unrelated setting.Blood samples were collected pre-transplant before and after(on day-1)the conditioning therapy and on days+2,+4,+6,+10,+20,and+30 after allo-HSCT.Serum levels of IL-2 and IFNgamma were determined using ELISA.RESULTS Patients were divided into four groups depending on the presence of acute GvHD and clinical manifestations of infection.Group I included patients with neither acute GvHD nor infections[n=15(24%)],group II consisted of patients with infections without acute GvHD[n=17(27%)],group III was comprised of patients with acute GvHD without infections[n=9(15%)],and group IV included patients with both acute GvHD and infections[n=21(34%)].IFN-gamma concentrations were higher in Group II than in other groups on days+20(P=0.014)and+30(P=0.008).Post-hoc tests showed lower concentrations of IFN-gamma on day+30 in groups I(P=0.039)and IV(P=0.017)compared to group II.The levels of IL-2 were mostly undetectable.CONCLUSION Serum levels of IFN-gamma following allo-HSCT progressively escalate.High ser

关 键 词：INTERLEUKIN-2 INTERFERON-GAMMA Cytokine profiles Acute myeloid leukemia Allogeneic hematopoietic stem cell transplantation Acute graft-versus-host disease 

分 类 号：R457.7[医药卫生—治疗学]