Effect of panel reactive antibodies on T cell immunity reinstatement following renal transplantation  

作　　者：Lampros Vagiotas Maria Stangou Efstratios Kasimatis Aliki Xochelli Grigorios Myserlis Georgios Lioulios Vasiliki Nikolaidou Manolis Panteli Konstantinos Ouranos Nikolaos Antoniadis Daoudaki Maria Aikaterini Papagianni Georgios Tsoulfas Asimina Fylaktou 

机构地区：[1]Department of Transplant Surgery,School of Medicine,Aristotle University of Thessaloniki,Hippokration General Hospital,Thessaloniki 54642,Greece [2]Department of Nephrology,School of Medicine,Aristotle University of Thessaloniki,Hippokration General Hospital,Thessaloniki 54642,Greece [3]Department of Immunology,National Peripheral Histocompatibility Center,Hippokration General Hospital,Thessaloniki 54642,Greece [4]Medical School Aristotle University of Thessaloniki,Biochemistry Laboratory,School of Medicine,Aristotle University of Thessaloniki,Thessaloniki 54124,Greece

出　　处：《World Journal of Transplantation》2022年第10期313-324,共12页世界移植杂志

摘　　要：BACKGROUND Chronic kidney disease is associated with immunological disorders,presented as phenotypic alterations of T lymphocytes.These changes are expected to be restored after a successful renal transplantation;however,additional parameters may contribute to this process.AIM To evaluate the impact of positive panel reactive antibodies(PRAs)on the restoration of T cell phenotype,after renal transplantation.METHODS CD4CD28null,CD8CD28null,natural killer cells(NKs),and regulatory T cells(Tregs)were estimated by flow cytometry at T0,T3,and T6 which were the time of transplantation,and 3-and 6-mo follow-up,respectively.Changes were estimated regarding the presence or absence of PRAs.RESULTS Patients were classified in two groups:PRA(-)(n=43)and PRA(+)(n=28)groups.Lymphocyte and their subtypes were similar between the two groups at T0,whereas their percentage was increased at T3 in PRA(-)compared to PRA(+)[23(10.9-47.9)vs 16.4(7.5-36.8)μ/L,respectively;P=0.03].Lymphocyte changes in PRA(-)patients included a significant increase in CD4 cells(P<0.0001),CD8 cells(P<0.0001),and Tregs(P<0.0001),and a reduction of NKs(P<0.0001).PRA(+)patients showed an increase in CD4(P=0.008)and CD8(P=0.0001),and a reduction in NKs(P=0.07).CD4CD28null and CD8CD28null cells,although initially reduced in both groups,were stabilized thereafter.CONCLUSION Our study described important differences in the immune response between PRA(+)and PRA(-)patients with changes in lymphocytes and lymphocyte subpopulations.PRA(+)patients seemed to have a worse immune profile after 6 mo follow-up,regardless of renal function.

关 键 词：Chronic kidney disease Panel reactive antibodies Lymphocyte subpopulation CD4CD28null cells CD8CD28null cells 

分 类 号：R73[医药卫生—肿瘤]