白药谷精草化学成分及抑菌活性研究  

Study on the chemical components and their antibacterial activities from Eriocaulon cinereum R.Br.

作　　者：徐冲[1] 王显婷 郭小红[1] 冷静[1] 陈玲[1] 谭道鹏 XU Chong;WANG Xian-ting;GUO Xiao-hong;LENG Jing;CHEN Ling;TAN Dao-peng(Institute of Traditional Chinese Medicine Preparation,Chongqing Hospital of Traditional Chinese Medicine/Chongqing Academy of Traditional Chinese Medicine,Chongqing 400021,China;School of Pharmacy,Zunyi Medical University,Zunyi 563000,China)

机构地区：[1]重庆市中医院/重庆市中医研究院中药制剂研究所,重庆400021 [2]遵义医科大学药学院,贵州遵义563000

出　　处：《中国药物化学杂志》2022年第9期700-706,共7页Chinese Journal of Medicinal Chemistry

基　　金：重庆市科卫联合中医药科研项目(2019ZY023205);重庆市临床药学重点专科建设项目(渝卫办发[2020]68号)。

摘　　要：目的研究白药谷精草(Eriocaulon cinereum R.Br.)乙醇提取物的化学成分,并进行体外抗菌活性评价。方法利用硅胶柱色谱、Sephadex LH-20柱色谱和制备高效液相色谱等手段进行分离纯化,根据理化性质和波谱数据对化合物进行结构鉴定。采用二倍微孔稀释法,对分离得到的化合物进行体外抗菌活性测试。结果与结论从白药谷精草中分离得到13个化合物,采用波谱分析技术鉴定为cassipourol(1)、α-菠甾醇(2)、莽草酸(3)、龙胆酸(4)、异阿魏酸(5)、野鸢尾黄素(6)、木犀草苷(7)、根皮苷(8)、高车前素-4′-葡萄糖苷(9)、异鼠李素-3-O-新橙皮苷(10)、槲皮苷(11)、高车前素(12)和金丝桃苷(13),化合物1为首次从该属植物中分得,化合物2~11为首次从该植物中分离得到。对以上化合物进行体外抗菌活性测试,发现酚酸类成分为其抑菌物质基础。Eriocaulon cinereum R.Br.is a herbal medicine widely applied in Sichuan-Chongqing area to treat conjunctivitis,corneal nebula,headaches and toothaches.To further develop this herb,chemical components and corresponding antibacterial activities were studied.Thirteen compounds were isolated and identified via chromatographic and spectral technologies as cassipourol(1),spinasterol(2),shikimic acid(3),gentisic acid(4),isoferulic acid(5),irigenin(6),luteoloside(7),phloridzin(8),hispidulin 4′-O-β-D-glucopyranoside(9),isorhamnetin-3-O-α-L-rhamnosyl(1→2)-β-D-glucopyranoside(10),quercitrin(11),hispidulin(12)and hyperoside(13).Compounds 1-11 were isolated from E.cinereum for the first time.Antibacterial activity assay in vitro suggested that phenolic acid derivates were its effective substances.

关 键 词：白药谷精草 化学成分 抑菌活性 

分 类 号：R284[医药卫生—中药学]