牛精浆中氨基酸含量与精子活力的关联性分析  

Correlation Analysis Between Amino Acid Content in Bovine(Bos taurus)Seminal Plasma and Sperm Motility

作　　者：李欣 韩碧莹 杨明[1] 张学丽 海超 李光鹏[1] 赵跃芳 LI Xin;HAN Bi-Ying;YANG Ming;ZHANG Xue-Li;HAI Chao;LI Guang-Peng;ZHAO Yue-Fang(State Key Laboratory of Reproductive Regulation and Breeding of Grassland Livestock,College of Life Science,Inner Mongolia University,Hohhot 010070,China)

机构地区：[1]内蒙古大学生命科学学院省部共建草原家畜生殖调控与繁育国家重点实验室,呼和浩特010070

出　　处：《农业生物技术学报》2022年第10期1894-1902,共9页Journal of Agricultural Biotechnology

基　　金：内蒙古自治区自然科学基金(2021MS03004);内蒙古自治区科技厅重大专项省部共建草原家畜生殖调控与繁育国家重点实验室自主课题(2019ZD031;2020ZD0008)。

摘　　要：精液冷冻保存在良种选育中发挥重要作用,但是精液冷冻会引起精子损伤。为了探寻精浆中决定精子冷冻耐受能力的关键因子,本研究采集了5头鲁西牛(Bos taurus)和5头蒙古牛的精液,系统地检测分析了精液体积、精子密度、精子活力等指标并通过HPLC技术定量分析了鲁西牛和蒙古牛的精浆中各氨基酸的含量。研究结果显示,10头牛的精液体积平均为4.85 m L,精子密度平均为13.76亿个/mL,鲜精和冻精活力分别为69.67%和35.68%,精浆中总氨基酸浓度为25~54 mg/mL,且蒙古牛高于鲁西牛;精浆中各氨基酸浓度与精子活力相关性分析结果表明,酪氨酸(Tyr)、苯丙氨酸(Phe)、丝氨酸(Ser)、精氨酸(Arg)、苏氨酸(Thr)、半胱氨酸(Cys)的浓度与冻精活力存在显著相关性,Pearson相关系数r值分别为0.743、0.755、0.662、0.649、0.725、0.729(P<0.05),脯氨酸(Pro)的浓度与冻精活力存在极显著相关性(r=0.768,P<0.01)。综上可知,精浆中氨基酸含量的检测可以作为牛精子冷冻耐受能力评价的潜在指标。本研究为冷冻精液质量的进一步提高提供了理论依据。Cryopreservation of semen plays a vital role in accelerating genetic improvement and elite breeding,but it has a detrimental effect on sperm quality.In order to explore the key factors related to sperm freezing tolerance in seminal plasma,in this study,the semen of 5 Luxi cattle(Bos taurus)and 5 Mongolian cattle were collected,semen volume,sperm density,sperm motility and other indicators were systematically detected and analyzed,and the contents of various amino acids in seminal plasma were quantitatively analyzed by HPLC.The results showed that the average semen volume of 10 cattle was 4.85 mL,the average sperm density was 1.376 billion pieces/mL,the vitality of fresh sperm and frozen sperm were 69.67%and 35.68%respectively,and the concentration of total amino acids in seminal plasma ranged from 25 to 54 mg/mL,and Mongolian cattle were higher than Luxi cattle;The correlation analysis between the concentration of amino acids in seminal plasma and sperm motility showed that the concentrations of Tyr,Phe,Ser,Arg,Thr and Cys were significantly correlated with frozen sperm motility,and Pearson correlation coefficient r values were0.743,0.755,0.662,0.649,0.725 and 0.729,respectively(P<0.05),and the concentration of Pro was extremely significantly correlated with frozen sperm motility(r=0.768,P<0.01).In conclusion,the detection of amino acid content in seminal plasma can be used as a potential index to evaluate the freezing tolerance of bovine sperm.This study provides theoretical basis for further improving the quality of frozen sperm.

关 键 词：牛精液 精浆 氨基酸 精子活力 精液冷冻 

分 类 号：S813[农业科学—畜牧学] S814.3[农业科学—畜牧兽医]