Integrative Proteomic Analysis of Multiple Posttranslational Modifications in Inflammatory Response  

作　　者：Feiyang Ji Menghao Zhou Huihui Zhu Zhengyi Jiang Qirui Li Xiaoxi Ouyang Yiming Lv Sainan Zhang Tian Wu Lanjuan Li 

机构地区：[1]State Key Laboratory for Diagnosis and Treatment of Infectious Diseases,National Clinical Research Center for Infectious Diseases,Collaborative Innovation Center for Diagnosis and Treatment of Infectious Diseases,The First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China [2]The First Affiliated Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China [3]Department of Colorectal Surgery,Sir Run Run Shaw Hospital,College of Medicine,Zhejiang University,Hangzhou 310003,China [4]Quzhou Second People’s Hospital,Quzhou 324000,China

出　　处：《Genomics, Proteomics & Bioinformatics》2022年第1期163-176,共14页基因组蛋白质组与生物信息学报（英文版）

基　　金：supported by the National Key R&D Program of China(Grant Nos.2016YFC1101304/3 and 2017YFC1200100);the National Natural Science Foundation of China(Grant No.81400589);the National S&T Major Project,China(Grant No.2017ZX10202202-001-008);the Science Fund for Creative Research Groups of the National Natural Science Foundation,China(Grant No.81721091)。

摘　　要：Posttranslational modifications(PTMs)of proteins,particularly acetylation,phosphorylation,and ubiquitination,play critical roles in the host innate immune response.PTMs’dynamic changes and the crosstalk among them are complicated.To build a comprehensive dynamic network of inflammation-related proteins,we integrated data from the whole-cell proteome(WCP),acetylome,phosphoproteome,and ubiquitinome of human and mouse macrophages.Our datasets of acetylation,phosphorylation,and ubiquitination sites helped identify PTM crosstalk within and across proteins involved in the inflammatory response.Stimulation of macrophages by lipopolysaccharide(LPS)resulted in both degradative and non-degradative ubiquitination.Moreover,this study contributes to the interpretation of the roles of known inflammatory molecules and the discovery of novel inflammatory proteins.

关 键 词：PROTEOME CROSSTALK Inflammation LIPOPOLYSACCHARIDE MACROPHAGE 

分 类 号：Q753[生物学—分子生物学]