Cytomorphological and genetic indicators in the early ontogenesis of the wild and farmed broad whitefish(Coregonus nasus)  

作　　者：Aleksandr Selyukov Oksana Zhigileva Leonid Shuman Svetlana Selyukova Vera Bogdanova 

机构地区：[1]Tyumen State University,Institute of Biology,Pirogov Street 3,Tyumen,625043,Russia [2]St.Petersburg Branch of the All-Russian Research Institute of Fisheries and Oceanography(St.Petersburg Branch of VNIRO),St.Petersburg,199155,Russia

出　　处：《Aquaculture and Fisheries》2023年第3期261-266,共6页渔业学报（英文）

基　　金：supported by RFBR grant No 19-44-890009.

摘　　要：The qualitative and quantitative composition of primordial germ cells(PGCs)and the genetic variability of embryos of the Coregonus nasus from the Ob river basin and domesticated population breeding outside the natural areal was examined.In the broad whitefish,typical,binuclear,polymorphic nuclei cells,as well as PGCs represented primary gonocytes during embryogenesis in clusters.When hatching larvae PGCs mitoses were noted only in domesticated juveniles.The genetic variability of embryos of the broad whitefish was studied by polymerase chain reaction of inter simple sequence repeats(ISSR-PCR).The high polymorphism of the broad whitefish cultured population was revealed.The proportion of polymorphic bands was 89.4%,Nei’s genetic diversity was 0.32.Reproduction of the domesticated broad whitefish did not affect the overall level of polymorphism of neutral DNA markers.These findings suggest the preservation of cytomorphological and genetic diversity in C.nasus embryo from different sources.

关 键 词：Broad whitefish Domestic brood stock Wild population EMBRYOGENESIS Genetic polymorphism Primordial germ cells 

分 类 号：S917.4[农业科学—水产科学]