银杏叶提取物对脑缺血再灌注损伤模型大鼠的脑保护作用  

Protective effect of ginkgo biloba extract on the brain in a cerebral ischemia-reperfusion injury rat model

作　　者：胡哲[1] 赵军 宋嵬[3] 闫旭升[3] 杨占君[3] 贾建新[3] HU Zhe;ZHAO Jun;SONG Wei;YAN Xusheng;YANG Zhanjun;JIA Jianxin(Health School of Baotou Medical College,Baotou 014050,China;Department of Neurosurgery,Third Clinical Hospital of Baotou Medical College,Baotou 014050;Department of Human Anatomy of Baotou Medical College,Baotou 014040)

机构地区：[1]包头医学院卫生健康学院解剖学教研室,内蒙古包头014050 [2]包头医学院第三临床医学院神经外科,内蒙古包头014050 [3]包头医学院人体解剖学教研室,内蒙古包头014040

出　　处：《中国比较医学杂志》2022年第9期55-61,共7页Chinese Journal of Comparative Medicine

基　　金：内蒙古自治区卫生计生委科研计划项目(201701080)。

摘　　要：目的 脑缺血再灌注损伤(cerebral ischemia-reperfusion injury,CIRI)严重影响神经功能,本实验计划探讨银杏叶提取物(ginkgo biloba extract,GBE)对CIRI模型大鼠神经功能是否具有改善作用及其相关机制。方法 闭塞大鼠大脑中动脉2 h,随后再灌注22 h制备CIRI大鼠模型,给予不同剂量(25、50、100 mg/(kg·d))的GBE腹腔注射15 d,全程给药结束后对各组动物进行神经功能评分,评价GBE对CIRI模型大鼠运动功能的影响;采用2,3,5-三苯基氯化四氮唑(TTC)染色评价GBE对脑梗死的影响;蛋白免疫印迹法检测自噬标志性蛋白和凋亡相关蛋白的表达量;ELISA法检测血清中谷胱甘肽过氧化物酶(glutathione peroxidase,GSH-px)和超氧化物歧化酶(superoxide dismutase,SOD)的活性以及丙二醛(malondialdehyde,MDA)的含量。结果 GBE可明显提高CIRI模型大鼠的神经功能评分,明显减小脑梗死体积;显著上调血清SOD与GSH-px的活性,以及降低MDA的含量;GBE可明显提高Beclin-1的蛋白表达量、LC3-II/LC3-I和Bcl-2/Bax的蛋白表达量比值,相反降低了Caspase-3的蛋白表达量。结论 GBE改善CIRI模型大鼠的运动功能障碍可能是通过抑制氧化应激与凋亡进而激活自噬系统,实现对脑细胞的保护作用。Objective The exact pathogenesis of cerebral ischemia-reperfusion injury(CIRI) remains unclear.This study explored whether ginkgo biloba extract(GBE) can improve neurological function in a CIRI rat model and the related mechanisms.Methods The CIRI rat model was induced by middle cerebral artery occlusion for 2 h,followed by reperfusion for 22 h.Varying dosages of GBE were administered intraperitoneally for 15 days.Subsequently,CIRI model rats were scored for neurological function to evaluate the effect of GBE on their motor function.In addition,2,3,5-triphenyltetrazolium chloride staining was used to detect the volume of infarcted brain,Western blot was used to detect expression of autophagy marker proteins and apoptosis-related proteins,the serum activities of superoxide dismutase(SOD) and glutathione(GSH-px) and serum level of malonaldehyde was detected by ELISA.Results GBE significantly improved the neurological function scores of CIRI model rats,markedly reduced cerebral infarction volumes,and significantly upregulated the activities of serum SOD and GSH-px,while decreasing the level of MDA.In addition,GBE significantly increased protein expression of Beclin-1 and protein expression ratios of LC3-II/LC3-I and Bcl-2/Bax,while remarkedly reducing protein expression of Caspase-3.Conclusions GBE improved the motor dysfunction of CIRI model rats by inhibiting oxidative stress and apoptosis,as well as activating the autophagy system to achieve a protective effect on brain cells.

关 键 词：脑缺血再灌注损伤 银杏叶提取物 自噬 凋亡 氧化应激 

分 类 号：R-33[医药卫生]