紫菀酮改善OVA诱导哮喘SD幼鼠的免疫反应及TLRs的表达  

Shionone improves immune responses and Toll-like receptor expression in ovalbumin-induced asthmatic rats

作　　者：艾奎 李桢 AI Kui;LI Zhen(Department of Pediatrics,Guanggu Hospital Area,Wuhan Third Hospital,Wuhan 430074,China)

机构地区：[1]武汉市第三医院光谷院区儿科,武汉430074

出　　处：《中国比较医学杂志》2022年第9期76-81,共6页Chinese Journal of Comparative Medicine

基　　金：武汉市卫健委科研项目(WZ21C52)。

摘　　要：目的 研究紫菀酮对卵清蛋白(onalbumin,OVA)诱导哮喘SD幼鼠的Toll样受体(Toll-like receptors,TLRs)表达和免疫反应的影响。方法 将24只SD幼鼠随机分为对照组、模型组、紫菀酮组和地塞米松组,每组6只。观察幼鼠行为学变化,苏木精-伊红(hematoxylin-eosin,HE)染色观察治疗后肺组织病理学情况,qPCR检测血浆中TLRs mRNA的表达量;ELISA检测血清中炎症细胞因子TNF-α、IL-1β、IL-6、IL-4、IL-13的表达水平。结果 模型组幼鼠呼吸急促,打喷嚏口鼻发紫,分泌粘液,亢奋易怒;支气管粘膜充血水肿,支气管上皮细胞脱落,气道及肺组织周围有大量炎症细胞浸润。紫菀酮和地塞米松用药后,幼鼠呼吸较为平缓,粘液分泌减少,亢奋易怒也有所减少,但地塞米松组出现了攻击性增强的现象;支气管粘膜充血水肿的程度有所缓解,气道及肺组织周围炎症细胞的数量也有所减少。与模型组比,紫菀酮组和地塞米松组TLR1、TLR9、TLR10 mRNA的表达量显著升高(P<0.01),TLR2、TLR3、TLR4、TLR5、TLR6、TLR7、TLR8 mRNA的表达量显著降低(P<0.01),TNF-α、IL-1β、IL-6、IL-4、IL-13的水平显著降低(P<0.01)。结论 紫菀酮能够改善OVA诱导的哮喘SD幼鼠肺组织的炎症情况,并能使TLRs的表达量产生变化,为后续研究紫菀酮对TLRs信号通路的作用机制打下了基础。Objective To investigate the effects of shionone on Toll-like receptor(TLR) expression and immune responses in ovalbumin(OVA)-induced asthmatic rats.Methods Twenty-four SD rats were randomly divided into four groups(six rats per group):control,model,shionone and dexamethasone.The behavioral changes of young mice were observed,as was their lung histopathology after hematoxylin and eosin staining.mRNA expression of TLRs in plasma was detected by qPCR,and serum expression levels of inflammatory cytokines tumor necrosis factor α(TNF-α),interleukin(IL)-1β,IL-6,IL-4 and IL-13 were detected by ELISA.Results Rats in the model group were short of breath,sneezed,displayed a purple nose and mouth,secreted mucus and were hyperactive and irritable.In addition,these rats exhibited bronchial mucosa hyperemia edema,bronchial epithelial cell death,and large numbers of infiltrating inflammatory cells in the airway and lung tissue.After treatment with shionone and dexamethasone,young mice breathed more easily and exhibited decreased mucus secretion and irritability;however,aggression was increased in the dexamethasone group.The degree of hyperemia and edema of bronchial mucosa was relieved,and numbers of inflammatory cells around the airway and lung tissue were reduced.Compared with the model group,mRNA expression levels of TLR1,TLR9 and TLR10 in shionone and dexamethasone groups were significantly increased(P<0.01),whereas mRNA expression levels of TLR2~TLR8 were significantly decreased(P<0.01).Levels of TNF-α,IL-1β,IL-6,IL-4 and IL-13 were significantly decreased(P<0.01).Conclusions Shionone can improve the immune response of OVA-induced SD rats and alter the expression of TLRs,laying the foundation for further study of the mechanism of shionone on TLR signaling pathways.

关 键 词：紫菀酮 哮喘 免疫反应 Toll样受体 

分 类 号：R-33[医药卫生]