茯苓提取物对1型糖尿病小鼠血糖及肠道菌群的调节作用  

Study on the mechanism of Poria cocos extract on the regulation of blood sugar and intestinal flora structure in type 1 diabetic mice

作　　者：刘蕾[1] 李海涛[1] 郑华月[1] 陈琼[2] LIU Lei;LI Haitao;ZHENG Huayue;CHEN Qiong(Department of Pediatrics,the First Affiliated Hospital of Nanyang Medical College,Nanyang 473000,China;Department of Endocrinology,Genetics and Metabolism,Zhengzhou Children s Hospital/He nan Children s Hospital/Children s Hospital Affiliated to Zhengzhou University,Zhengzhou 450053,China)

机构地区：[1]南阳医学高等专科学校第一附属医院儿科,南阳473000 [2]郑州儿童医院/河南省儿童医院/郑州大学附属儿童医院内分泌遗传代谢科,郑州450053

出　　处：《西北药学杂志》2022年第6期89-94,共6页Northwest Pharmaceutical Journal

基　　金：2018年河南省医学科技攻关计划项目(编号:2018020597)。

摘　　要：目的 研究茯苓提取物(PCE)对1型糖尿病(T1DM)小鼠血糖及肠道菌群结构的调节作用及其可能机制。方法 60只雄性昆明种小鼠,随机取12只作为对照组,另外48只用链脲佐菌素建立T1DM小鼠模型,造模成功43只,分为模型组(11只)、PCE低剂量组(11只)、PCE高剂量组(11只)、二甲双胍组(10只),予以对应灌胃干预,连续28 d,干预后分别测定各组小鼠空腹血糖(FPG)、血清胰岛素(INS)、胰高血糖素(GC)水平,HE染色观察小鼠胰腺组织形态学,ELISA法检测结肠组织细胞IL-10及IL-17水平,测定结肠Th17细胞、Treg细胞比例与结肠内容物肠道菌群丰度。结果 模型组、PCE低剂量组、PCE高剂量组、二甲双胍组FPG、血清GC、结肠组织IL-17、Th17细胞比例及肠道菌群拟杆菌门比例高于对照组,血清INS、结肠组织IL-10、Treg细胞比例、肠道菌群乳酸杆菌属及瘤胃杆菌科比例低于对照组(P<0.05);PCE低剂量组、PCE高剂量组、二甲双胍组FPG、血清GC、结肠组织IL-17、Th17细胞比例及肠道菌群拟杆菌门比例低于模型组,血清INS、结肠组织IL-10、Treg细胞比例、肠道菌群乳酸杆菌属及瘤胃杆菌科比例高于模型组(P<0.05);PCE高剂量组、二甲双胍组FPG、血清GC、结肠组织IL-17、Th17细胞比例及肠道菌群拟杆菌门比例低于PCE低剂量组,血清INS、结肠组织IL-10、Treg细胞比例、肠道菌群乳酸杆菌属及瘤胃杆菌科比例高于PCE低剂量组(P<0.05);PCE高剂量组和二甲双胍组上述指标比较差异无统计学意义。不同剂量PCE干预T1DM小鼠,胰岛细胞排列相对紧密,空泡减少,胰腺组织损伤有不同程度减轻,高剂量PCE损伤减轻程度更明显。结论 PCE用于T1DM小鼠,可降血糖,促进INS释放,降低GC水平,其作用机制可能是PCE可调节肠道菌群结构提高机体免疫、减轻炎症症状和调节血糖。Objective To study the mechanism of Poria cocos extract(PCE) on the regulation of blood glucose and intestinal flora structure in type 1 diabetes(T1 DM) mice.Methods 60 male Kunming mice were selected, 12 mice were randomly selected as the control group, and the others were modeled with streptozotocin. 43 mice were successfully modeled and divided into model group(11) and PCE low-dose group(11), PCE high-dose group(11), and metformin group(10). The corresponding intragastric intervention was given for 28 consecutive days. After the intervention, the levels of fasting plasma glucose(FPG), serum insulin(INS) and glucagon(GC) of each group were measured. The morphology of mouse pancreas was observed by HE staining, and IL-10 and IL-of colon tissue cells were detected by ELISA. The proportion of Th17 cells and Treg cells in colon and the abundance of intestinal flora of colon contents were measured.Results The FPG, GC, colon tissue IL-17, Th17 cell ratio, and intestinal flora bacteroides ratio of model group, PCE low-dose group, PCE high-dose group, and metformin group were higher than control group, while the INS, colon tissue IL-10, the proportion of Treg cells, the proportion of intestinal flora ratio of Lactobacillus and Rumenbacteriaceae were lower than control group(P<0.05). The FPG, GC, colon tissue IL-17, Th17 cell ratio, and the intestinal flora bacteroides ratio of PCE low-dose group, PCE high-dose group, metformin group were lower than model group, while the INS, colon tissue IL-10, Treg cell ratio, intestinal flora ratio of Lactobacillus and Rumenobacteria were higher than the model group(P<0.05). The FPG, serum GC, colon tissue IL-17, Th17 cell ratio, intestinal flora bacteroides ratio of PCE high-dose group, metformin group were lower than PCE low-dose group, while the INS, colon tissue IL-10, the proportion of Treg cells, the proportion of intestinal flora ratio of Lactobacillus and Rumenbacteriaceae were higher than the PCE low-dose group(P<0.05). Compared with PCE high-dose group and metformin group,

关 键 词：茯苓提取物 1型糖尿病 血糖 胰岛素 肠道菌群 炎性因子 免疫细胞 

分 类 号：R965[医药卫生—药理学]