酸性矿山废水中锰氧化菌的分离鉴定及其对Mn^(2+)的去除作用  

Isolation and identification of manganese oxidizing bacteria from acid mine wastewater and its effect on the removal of Mn^(2+)

作　　者：陈明珠 张林义 岳正波[1,2] 潘鑫 王绍平[3] 李伟 罗长海 刘兵 王进 CHEN Mingzhu;ZHANG Linyi;YUE Zhengbo;PAN Xin;WANG Shaoping;LI Wei;LUO Changhai;LIU Bing;WANG Jin(School of Resources and Environmental Engineering,Hefei University of Technology,Hefei 230009;Key Laboratory of Nanominerals and Pollution Control of Anhui Higher Education Institutes,Hefei University of Technology,Hefei 230009;Nanshan Mining Company Ltd,Anhui Maanshan Iron and Steel Mining Resources Group,Maanshan 243000;Anhui Magang Mining Resources Group Co.,Ltd.Ecological Restoration Technology Branch,Maanshan 243000)

机构地区：[1]合肥工业大学资源与环境工程学院,合肥230009 [2]合肥工业大学纳米矿物与污染控制安徽省高等学校重点实验室,合肥230009 [3]安徽马钢矿业资源集团南山矿业有限公司,马鞍山243000 [4]安徽马钢矿业资源集团有限公司生态修复科技分公司,马鞍山243000

出　　处：《环境科学学报》2022年第9期30-39,共10页Acta Scientiae Circumstantiae

基　　金：国家自然科学基金资助项目(No.U20A20325,41772361)。

摘　　要：含锰(Mn)重金属废水的处理是行业痛点与难点,近年来高效的生物脱锰技术得到广泛关注,但具有较高锰氧化效率菌株的报道仍然有限.本研究从酸性矿山废水中分离纯化出一株锰氧化细菌,并深入探究其微生物特性以及对Mn^(2+)的去除作用.通过生理生化及16S rRNA序列分析鉴定该菌株为短芽孢杆菌,并命名为Brevibacillus brevis MM2.进一步通过单因素实验探究了环境因子和初始锰浓度对菌株MM2的生长和锰去除率的影响.结果表明,菌株MM2对Mn^(2+)的最高耐受浓度可达1000 mg·L^(-1),且在弱酸条件下(pH 6、pH 5)仍然显现出良好的生长趋势,具有较高的锰去除率(91.93%,pH=6).此外,菌株MM2在30~35℃,pH 7,转速180 r·min^(-1)条件下生长趋势最好,当初始Mn^(2+)浓度为100 mg·L^(-1)时,Mn^(2+)的最高去除率为92.76%.最后,通过扫描电子显微镜(SEM)、X射线光电子能谱(XPS)、X射线衍射(XRD)、傅里叶红外光谱仪(FTIR)对代谢固相产物分析表明,菌株MM2介导生成的生物氧化锰附着在菌株表面并以层状和颗粒状两种形态呈现,锰平均氧化度为3.55,其成分主要为无定形的Mn_(2)O_(3)和MnO_(2).综上所述,本研究分离纯化的菌株MM2不仅能在弱酸性条件下生长且对锰具有较高的耐受和去除能力,在含锰废水的处理中具有较好应用前景.The treatment of heavy metal wastewater containing manganese(Mn)is an industrial difficulty.In recent years,efficient biological manganese removal has received a wide attention,but the strains with high manganese oxidation efficiency are still limited.In this study,a manganese oxidizing bacterium was isolated from acid mine drainage,and the microbial characteristics and Mn^(2+)removal were investigated.The strain was identified as Brevibacillus brevis by physiological,biochemical and 16S rRNA gene sequence analysis,and was named as Brevibacillus brevis MM2.The effects of environmental factors and initial manganese concentration on the growth and manganese removal rate of strain MM2 were further studied through single factor experiment.The results suggested that the maximum tolerance concentration of the strain MM2 to Mn^(2+)was up to 1000 mg·L^(-1),and still showed a good growth with a high manganese removal rate(91.93%,pH=6)under weak acid conditions(pH 5 and 6).In addition,the strain MM2 had the best growth under the conditions of 30~35℃,pH 7 and rotating speed of 180 r·min^(-1).When the initial Mn^(2+)concentration was 100 mg·L^(-1),the highest manganese removal rate of 92.76%was obtained.Finally,the metabolic solid phase products were analyzed by the scanning electron microscopy(SEM),X-ray photoelectron spectroscopy(XPS),X-ray diffraction(XRD),Fourier transform infrared spectroscopy(FTIR).The biological manganese oxide produced by the strain MM2 was attached to the surface of the strain in two forms,lamellar and granular,and its composition was mainly amorphous Mn_(2)O_(3) and MnO_(2),with an average Mn oxidation number of 3.55.In summary,the strain can grow under weakly acidic conditions,and has efficient manganese tolerance and removal capabilities,with great application potential for the treatment of manganesecontaining wastewater.

关 键 词：锰氧化细菌 酸性矿山废水 筛选鉴定 生物除锰 生物氧化锰 

分 类 号：X172[环境科学与工程—环境科学]