LOX和E-cadherin在口腔鳞癌组织中的表达及其临床相关性研究  

Study on the Expression of LOX and E-Cadherin Protein and their Clinical Correlation with Oral Squamous Cell Carcinoma.

作　　者：韩新生 贾佳欣 陈方园 张兴安 明华伟 袁宗毅 王华东 谭小尧 Han Xinsheng;Jia Jiaxin;Chen Fangyuan(Department of Oral and Maxillofacial Surgery,Nanchong Central Hospital,The Second Clinical Medical College of North Sichuan Medical College,Nanchong,Sichuan 637000,China.)

机构地区：[1]南充市中心医院·川北医学院第二临床医学院口腔颌面外科,四川南充637000

出　　处：《四川医学》2022年第9期873-877,共5页Sichuan Medical Journal

基　　金：川北医学院校级科研发展基金(编号:CBY20-QA-Y11);南充市科技局2020年市校科技战略合作专项基金(编号:20SXQT0096);川北医学院课程思政教学改革课题专项基金(编号:kcsz-040)。

摘　　要：目的检测赖氨酰氧化酶(LOX)蛋白和钙黏附蛋白E(E-cadherin)在口腔鳞状细胞癌(OSCC)组织中表达情况,并研究其表达与OSCC临床病理参数的相关性及两者的相互关系,探讨其在OSCC发生、发展中的作用。方法收集医院2017年1月至2019年12月住院手术切除明确诊断为OSCC的癌组织和其配对的癌旁正常组织标本80例,免疫组织化学技术检测癌组织和癌旁正常组织LOX、E-cadherin蛋白表达水平。结果LOX在癌组织的阳性表达明显高于癌旁正常组织,差异有统计学意义(P<0.05);E-cadherin在癌组织的阳性表达明显低于癌旁正常组织,差异有统计学意义(P<0.05)。LOX在癌组织中的高表达、E-cadherin在癌组织中的低表达与OSCC临床分期、淋巴结转移呈正相关(P均<0.05);与患者年龄、性别、肿瘤大小以及病理学分级无关(P均>0.05),并且LOX和E-cadherin在OSCC癌组织中的表达呈负相关(r=-0.478,P<0.05)。结论LOX、E-cadherin在OSCC癌组织和癌旁组织存在差异表达,并且其差异表达和OSCC临床分期、淋巴结转移呈正相关。LOX和E-cadherin在OSCC中的表达呈负相关,LOX可能通过相关通路下调E-cadherin的表达,从而促进OSCC上皮-间质转化的发生。因此,LOX在OSCC癌组织中高表达和E-cadherin的低表达可能是促进OSCC侵袭和转移的一个危险因素;LOX和E-cadherin表达水平可作为判断OSCC预后的标志物。Objective To detect LOX and E-cadherin in OSCC,and correlations between theirs expression with clinicopathological parameters of OSCC,and then explore theirs role relationship between OSCC occurrence and development.Methods From January 2017 to December 2019,80 cases of cancer tissue definitively diagnosed as OSCC and theirs paired paracancer normal tissue specimens were collected and detected by Immunohistochemistry(IHC),and detected LOX and E-cadherin expression in cancer tissues and adjacent normal tissues.Results Positive expression of LOX in cancer tissues was significantly higher than that in adjacent normal tissues with statistically significant difference(P<0.05).Positive expression of E-cadherin in cancer tissues was significantly lower than that in adjacent normal tissues with statistically significant difference(P<0.05).High expression of LOX and low expression of E-cadherin were positively correlated with OSCC clinical stage and lymph node metastasis(P<0.05).There was no correlation with age,sex,tumor size and pathological grade(P>0.05).LOX and E-cadherin in OSCC cancer tissues was negatively correlated(r=-0.478,P<0.05).Conclusion LOX and E-cadherin were differentially expressed in OSCC and normal tissues,differentially expressed LOX and E-cadherin were positively correlated with OSCC in clinical stage and lymph node metastasis.LOX and E-cadherin were negatively correlated in OSCC,and LOX may down-regulate E-cadherin through related pathways,thus promoting the occurrence of EMT in OSCC.Therefore,high expression of LOX and low expression of E-cadherin in OSCC cancer tissues may be a risk factor that promote invasion and metastasis of OSCC,and expressions of LOX and E-cadherin can be used as prognostic markers of OSCC.

关 键 词：口腔鳞癌 赖氨酰氧化酶 钙黏附蛋白E 上皮-间质转化 免疫组织化学技术 

分 类 号：R739.8[医药卫生—肿瘤]