High-density genetic mapping identified a major locus for environmental sex expression in pumpkin(Cucurbita moschata Duch.)  

作　　者：Hafiz Muhammad Khalid Abbas Hexun Huang Tingquan Wu Rui Wang Hu Du Sen Lu Shudan Xue Chunpeng Yao Qingmin Jin Yujuan Zhong 

机构地区：[1]Guangdong Key Laboratory for New Technology Research of Vegetables,Vegetable Research Institute,Guangdong Academy of Agricultural Sciences,Guangzhou 510640,China

出　　处：《Horticultural Plant Journal》2022年第5期593-601,共9页园艺学报（英文版）

基　　金：supported by Key Realm R&D Program of Guangdong Province(Grant No.2020B020220003);the National Natural Science Foundation of China(Grant No.31601748);the Agricultural competitive industry discipline team building project of Guangdong Academy of Agricultural Sciences(Grant No.202103TD).

摘　　要：Long-day length and high temperature inhibit sex expression in pumpkin(Cucurbita moschata Duch.),and therefore directly impact the production potential.In this study,female flowering patterns in photoperiod-insensitive(PPIS)and photoperiod-sensitive(PPS)germplasms differed significantly in a moderately long day and high temperature environment.However,both germplasms exhibited a similar response in short day with either low temperature or high temperature environment.Photoperiod sensitivity led to this difference in sex expression between the germplasms.For the traits of 1st female flowering node(FFFN)and number of female flowers(NFF),high-density linkage map construction and quantitative trait locus(QTL)mapping were performed using SLAF-seq technology and 162 F_(2) individuals generated from PPIS and PPS.In total,4655 SLAFs were selected and mapped on 20 linkage groups(LGs).The total map length was 2502.01 cM with an average interval distance of 0.75 cM.Major QTLs for both FFFN and NFF were detected on LG6 with intervals of 7.89 and 17.67 cM and PVE values of 30.5%and 22.9%,respectively.Further analyses of the major locus for FFFN revealed 73 protein-coding genes.Among them,4 were related to sex expression,photoperiod flowering,and hormone response.An InDel(insertion and deletion)marker partially correlated with FFFN of the F_(2) population was also developed.Our study identified the QTL for the sex expression response to environmental factors using the high-density linkage map.The identified candidate genes and markers will provide useful information about the molecular interaction between the environment and sex expression and for marker-assisted selection of pumpkin environment-insensitive resources.

关 键 词：PUMPKIN Photoperiod sensitivity Sex expression High-density genetic map SLAF-seq QTL mapping 

分 类 号：S642.1[农业科学—蔬菜学]