Transcriptomic and metabolomic analyses reveal that exogenous strigolactones alleviate the response of melon root to cadmium stress  

作　　者：Xuemiao Chen Xueyin Shi Qing Ai Jinying Han Huaisong Wang Qiushi Fu 

机构地区：[1]Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture and Rural Affairs,Institute of Vegetables and Flowers,Chinese Academy of Agricultural Sciences,Beijing 100081,China

出　　处：《Horticultural Plant Journal》2022年第5期637-649,共13页园艺学报（英文版）

基　　金：supported by the National Key Research and Development ProgramofChina(GrantNo.2020YFD1000300);China Agriculture Research System of MOF and MARA(Grant No.CARS-25);the Agricultural Science and Technology Innovation Program of the Chinese Academy of Agricultural Sciences(Grant No.CAASASTIP-IVFCAAS);Central Public-interest Scientific Institution Basal Research Fund(Grant No.IVF-BRF2022006).

摘　　要：Strigolactones(SLs)are classified into plant hormones,playing a key role as a mediator of plant growth in response to several abiotic stresses.Cadmium(Cd),a common heavy metal and soil pollutant,can suppress plant growth and development.In this work,we explored the effects of exogenous SLs on root formation in response to Cd stress using melon seeds subjected to seven germination treatments:CK(control),Cd(300 μmol·L^(-1) CdCl_(2)),and SL1-SL5(CdCl_(2)-stressed seeds pretreated with 0.1,0.5,1,2,and 3 μmol·L^(-1) GR24 solutions).The results indicated that SLs increased the antioxidant enzyme activities and root vigor and decreased the malondialdehyde(MDA)contents in the roots of Cdstressed melon seedlings.Then we used transcriptomic and metabolomic analyses to explore the mechanisms by which exogenous SLs protect against Cd stress.There were 242 significant differentially expressed genes(DEGs)(78 upregulated,164 downregulated)and 247 significantly differentially expressed metabolites(DEMs)(222 upregulated,25 downregulated)between the Cd and SL3 treatments.SLs altered the expression of genes related to redox formation processes,including peroxidase(POD),lipoxygenase(LOX),glutamate dehydrogenase(GDH),and glutathione S-transferase(GST).In addition,we found that SLs regulated the expression of the MYB,AP2/ERF,bHLH,and WRKY transcription factor families.The combined transcriptomic and metabolomic analyses revealed that the DEGs and DEMs involved in Cd stress alleviation were mainly related to the gene expression of jasmonic acid(JA)and flavonoid biosynthesis.SLs might induce LOX-related genes to regulate JA biosynthesis.Moreover,SLs might promote flavonoid biosynthesis by regulating eleven flavonoid-related genes and eight metabolites.The results provide a new perspective for studying the adaptation of plants to Cd stress.

关 键 词：MELON STRIGOLACTONES Cd stress ROOT Transcriptome METABOLOME 

分 类 号：S652[农业科学—果树学]