ATP2B1-eNOS通路基因多态性与生活方式对青少年血压偏高的交互作用  

Interaction of ATP2B1-eNOS pathway gene polymorphisms and lifestyle factors on high blood pressure among adolescents

作　　者：黄泽华 谢铭 肖田莉 曾媛 袁姝倩 龚悦 毛彬 郑婵娟[1] 阳益德 HUANG Zehua;XIE Ming;XIAO Tianli;ZENG Yuan;YUAN Shuqian;GONG Yue;MAO Bin;ZHENG Chanjuan;YANG Yide(Department of Child and Adolescent Health,School of Medicine,Hunan Normal University,Changsha(410013),China)

机构地区：[1]湖南师范大学医学院儿童少年卫生学教研室,长沙410013 [2]长沙市第三医院科教科 [3]湖南师范大学医学院分子流行病学湖南省重点实验室 [4]湖南师范大学医学人文中心

出　　处：《中国学校卫生》2022年第9期1407-1411,共5页Chinese Journal of School Health

基　　金：国家自然科学基金(81903336);湖南省自然科学基金(2019JJ50376)。

摘　　要：目的探索ATP2B1-eNOS通路基因多态性对血压表型的基因-生活方式交互作用,为阐明青少年血压偏高的发生机制提供线索。方法采用方便整群抽样设计,于2019年7—8月对3所初中872名学生进行问卷调查、人体指标测量和血液样本收集。在外周血液样本提取DNA后,对基因多态性(ATP2B1/rs17249754和rs2070759,eNOS/rs1799983和rs2070744)进行基因分型。采用Logistic回归分析基因多态性与血压表型之间的关联并纳入交互项分析基因多态性与生活方式交互作用。结果青少年血压偏高的检出率为9.52%(男生9.15%,女生9.87%),性别差异无统计学意义(χ^(2)=0.13,P=0.72)。男生和女生之间年龄、收缩压、舒张压、体质量指数(BMI)分类、出生体重、每日在校体育锻炼时间与每日在家玩电子游戏时间差异均有统计学意义(P值均<0.05)。Logistic回归分析显示,eNOS/rs2070744多态性在隐性模型下与血压偏高存在关联,且CC基因型携带者相较于TT/TC基因型携带者血压偏高的发生风险升高(OR=3.88,95%CI=1.00~15.02,P<0.05)。基因-生活方式交互作用结果显示,ATP2B1/rs2070759多态性与在校体育锻炼时间对血压偏高存在交互作用(P_(交互)=0.05)。在每日在校体育锻炼时间<1 h亚组中,TT/TG基因型携带者相较于GG基因型携带者会增加血压偏高发生风险(OR=2.65,95%CI=1.11~6.30,P<0.05);而每日在校体育锻炼时间≥1 h亚组中,与血压偏高无相关性。结论eNOS/rs2070744多态性与青少年血压偏高相关,ATP2B1/rs2070759多态性与在校体育锻炼时间对血压偏高存在交互作用。青少年应增加在校体育锻炼时间,这将有助于高血压易感者维持正常的血压水平。Objective To explore the gene-lifestyle interaction of ATP2B1-eNOS pathway gene polymorphisms on blood pressure.Methods Using the convenient cluster sampling method,a total of 872 junior middle school students from 3 school in July to August 2019,were included in the final analysis.The survey included questionnaire investigation,anthropometry measurement and blood sample collection.After DNA was extracted from peripheral blood samples,the gene polymorphisms(ATP2B1 gene rs17249754 and rs2070759,eNOS gene rs1799983 and rs2070744)were genotyped.Logistic regression model was used to analyze the association between gene polymorphism and blood pressure phenotypes.Results The prevalence of high blood pressure was 9.52%in adolescents(9.15%in boys and 9.87%in girls),with no significant sex difference(χ^(2)=0.13,P=0.72).There were statistically significant differences between boys and girls in age,systolic blood pressure(SBP),diastolic blood pressure(DBP),body mass index(BMI)classification,birth weight,daily school physical exercise time and daily playing video games time(P<0.05).Logistic regression analysis showed that,eNOS gene rs2070744 polymorphism was associated with high blood pressure(HBP)under the recessive model,and the risk of HBP in CC genotype carriers were higher than that TT/TC genotype carriers(OR=3.88,95%CI=1.00-15.02,P<0.05).The results of gene-lifestyle interaction showed that ATP2B1 gene rs2070759 polymorphism gene had an interaction with the time of physical exercise in school(P_(interaction)=0.05).In the subgroup with daily physical exercise time at sch-ool<1 hour,the TT/TG genotype carriers were associated with increased risk of HBP compared with GG genotype carriers(OR=2.65,95%CI=1.11-6.30,P<0.05).But in the subgroup with daily physical exercise time in school≥1 hour,rs2070759 was not significantly associated with HBP.Conclusion eNOS/rs2070744 polymorphisms are associated with risk of HBP among adolescents.There is significant interaction between ATP2B1 gene rs2070759 polymorphism and physical exe

关 键 词：基因 血压 回归分析 青少年 

分 类 号：R446[医药卫生—诊断学] R179[医药卫生—临床医学] R725.4