心肌梗死相关转录本对视网膜母细胞瘤影响机制的研究  

Effect of myocardial infarction-associated transcripts on retinoblastoma

作　　者：蒋瑜[1] 孙先桃 孙爽[1] 卢跃兵 JIANG Yu;SUN Xian-tao;SUN Shuang;LU Yue-bing(Department of Ophthalmology,Children's Hospital of Zhengzhou University,Henan Children's Hospital,Zhengzhou Children's Hospital,Zhengzhou,Henan 450000,China)

机构地区：[1]郑州大学附属儿童医院河南省儿童医院郑州儿童医院眼科,河南郑州450000

出　　处：《中华全科医学》2022年第10期1650-1653,1736,共5页Chinese Journal of General Practice

基　　金：河南省医学科技攻关计划联合共建项目(LHGJ 20190952)。

摘　　要：目的探讨心肌梗死相关转录本(MIAT)靶向miR-145对视网膜母细胞瘤增殖和凋亡的影响。方法选取2015年9月—2020年9月在郑州大学附属儿童医院因视网膜母细胞瘤行眼球摘除患儿30例,收集患儿肿瘤组织及癌旁组织。培养人视网膜母细胞瘤细胞Y79,按照随机数字表法分为si-NC组、si-MIAT组、miR-NC组、miR-145组、si-MIAT+anti-miR-NC组、si-MIAT+anti-miR-145组。采用RT-qPCR检测细胞MIAT和miR-145的表达;CCK-8检测细胞增殖;流式细胞术检测细胞凋亡;Western blotting检测CyclinD1、p21、Bcl-2、Bax、p-PI3K、p-Akt蛋白表达;双荧光素酶报告检测MIAT与miR-145的靶向关系。结果与癌旁组织比较,视网膜母细胞瘤组织中MIAT表达显著上调(3.61±0.28 vs.1.05±0.11),miR-145表达显著下调(0.33±0.03 vs.1.03±0.09,均P<0.05)。与si-NC组比较,si-MIAT组细胞活力显著下调;细胞凋亡率显著上调;p-PI3K蛋白、p-Akt蛋白表达显著下调(均P<0.05)。与miR-NC组比较,miR-145组细胞活力显著下调;细胞凋亡率显著上调(均P<0.05)。与si-MIAT+anti-miR-NC组比较,si-MIAT+anti-miR-145组细胞活力显著上调;细胞凋亡率显著下调;p-PI3K蛋白、p-Akt蛋白表达显著上调(均P<0.05)。结论抑制MIAT表达、上调miR-145表达可促进视网膜母细胞瘤凋亡,抑制增殖,其机制与PI3K/Akt通路有关。Objective To explores the effect of lncRNA MIAT targeting miR-145 on the proliferation and apoptosis of retinoblastoma.Methods From September 2015 to September 2020,30 children with retinoblastoma underwent enucleation in Children's Hospital of Zhengzhou University were selected,and tumor tissues and adjacent tissues were collected.Human retinoblastoma cells(Y79)were cultured and divided into si-NC,si-MIAT,miR-NC,miR-145,si-MIAT+anti-miR-NC and si-MIAT+anti-miR-145 groups according to the random number table method.RT-qPCR was used in detecting the expression levels of MIAT and miR-145 in the cells.CCK-8 was used in detecting cell proliferation,and flow cytometry was used in detecting cell apoptosis.Cyclin D1,p21,Bcl-2,Bax,p-PI3K and p-Akt protein expression levels were detected with Western blotting,and dual luciferase reporter was used in detecting the targeted relationship between MIAT and miR-145.Results Compared with adjacent tissues,the expression of MIAT in retinoblastoma tissue was significantly up-regulated,and the expression of miR-145 was significantly down-regulated(3.61±0.28 vs.1.05±0.11,0.33±0.03 vs.1.03±0.09,all P<0.05).Compared with the si-NC group,cell viability in the si-MIAT group was significantly down-regulated,mortality rate was significantly up-regulated,and p-PI3K protein and p-Akt protein expression were significantly down-regulated(all P<0.05).Compared with the miR-NC group,cell viability in the miR-145 group showed significantly down-regulated,apoptosis rate was significantly up-regulated(all P<0.05).Compared with the si-MIAT+anti-miR-NC group,cell viability was significantly up-regulated in the si-MIAT+anti-miR-145 group,cell apoptosis rate was significantly down-regulated,whereas the expression levels of p-PI3K and p-Akt proteins were significantly up-regulated(all P<0.05).Conclusion The inhibition of MIAT expression and up-regulation of miR-145 expression can promote apoptosis and inhibit the proliferation of retinoblastoma.The mechanism is related to the PI3K/Akt pathway.

关 键 词：长链非编码RNA MIAT 微小RNA-145 磷脂酰肌醇-3激酶/蛋白激酶B信号通路 视网膜母细胞瘤细胞 增殖 凋亡 

分 类 号：R739.72[医药卫生—肿瘤]