乳腺肿瘤冷冻切片和石蜡切片中RAGE和Ki-67的差异表达及其意义  

The significant implications of differential expressions of RAGE and Ki-67 in frozen and paraffin sections of breast cancer tissues

作　　者：李江伟[1] 李贞[1] 夏雨涵 黄培[1] 李振乾[1] 秦慧[1] 高冬玲[1] Li Jiangwei;Li Zhen;Xia Yuhan;Huang Pei;Li Zhenqian;Qin Hui;Gao Dongling(Department of Pathology,The First Affiliated Hospital of Zhengzhou University,Zhengzhou 450052,China)

机构地区：[1]郑州大学第一附属医院病理科,郑州450052

出　　处：《中国组织化学与细胞化学杂志》2022年第4期417-420,共4页Chinese Journal of Histochemistry and Cytochemistry

摘　　要：目的探索乳腺肿瘤组织冷冻切片和石蜡切片中RAGE和Ki-67的表达是否存在差异及启示意义。方法对15例乳腺肿瘤切除标本的冷冻切片和相对应的石蜡切片行晚期糖基化终末产物受体(receptor for advanced glycation end product,RAGE)和Ki-67的免疫组织化学SP法染色,分别分析两者在染色阳性率和强度上的差异。结论乳腺肿瘤组织冷冻切片的RAGE和Ki-67免疫组织化学染色阳性率和强度较其石蜡组织切片低,使用冷冻切片做免疫组织化学染色时需要探索每种组织、抗原最佳的实验条件。Objective To explore the different expressions of receptor for advanced glycation end product(RAGE)and Ki-67 in frozen and paraffin sections of breast cancer tissues,and its implications.Methods The frozen and paired paraffin sections of 15 breast cancer specimens were stained with RAGE and Ki-67 by immunohistochemical SP method,and the changes of staining rates and in-tensity were analyzed.Conclusion The positive staining rates and intensity of RAGE and Ki67 in frozen sections of breast cancer was lower than that in paraffin sections,and exploration of optimal experimental conditions for each antigen and tissue were required when using frozen sections.

关 键 词：RAGE KI-67 乳腺肿瘤 冷冻切片 石蜡切片 

分 类 号：R361[医药卫生—病理学]