单细胞荟萃分析SARS-CoV-2感染后T淋巴细胞在各个器官中的功能差异  

Single-cell meta-analysis of T lymphocytes functional differences in various organs after SARS-CoV-2 infection

作　　者：童霞 张华 彭玲[1,2] 张鑫[2,4] 张兰兰[1] TONG Xia;ZHANG Hua;PENG Ling;ZHANG Xin;ZHANG Lanlan(Department of Respiratory and Critical Care Medicine,West China Hospital,Chengdu,Sichuan 610041,P.R.China;West China(Airport)Hospital of Sichuan University(The First People's Hospital of Shuangliu District,Chengdu),Chengdu,Sichuan 610200,P.R.China;West China Second University Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China;Division of Gastroenterology,West China Hospital,Sichuan University,Chengdu,Sichuan 610041,P.R.China)

机构地区：[1]四川大学华西医院呼吸与危重症医学科,四川成都610041 [2]四川大学华西空港医院(成都市双流区第一人民医院),四川成都610200 [3]四川大学华西第二医院,四川成都610041 [4]四川大学华西医院消化内科,四川成都610041

出　　处：《中国呼吸与危重监护杂志》2022年第5期330-339,共10页Chinese Journal of Respiratory and Critical Care Medicine

基　　金：(人社部)人事处博士后经费-博士后新冠疫情防控专项基金(0040204153344)。

摘　　要：目的了解SARS-CoV-2感染后T淋巴细胞在各个器官中的功能差异。方法应用生物信息学方法,利用高通量基因表达数据库数据(GSE171668、GSE159812、GSE159556、GSE167747)及单细胞技术的分析方法,分析SARS-CoV-2感染患者各器官的T淋巴细胞在体内的功能差异。结果通过对新冠肺炎患者16个肝脏、19个心脏、2个脾脏、6个大脑、58个肺、21个肾脏、5个胰腺组织的单细胞数据提取,发现入侵基因在肺和胰腺的T淋巴细胞中表达相对较高。肺的T淋巴细胞高表达干扰素信号通路,而其他器官表达相对较低;同时肺的T淋巴细胞也高表达脂肪酸结合位点。结论SARS-CoV-2感染后,相对于其他器官,肺的T淋巴细胞具有特殊的干扰素活化信号通路和脂肪酸结合位点。Objective To explore the functional heterogeneity of T lymphocytes in various organs after SARSCoV-2 infection. Methods Using the public database GEO data(GSE171668, GSE159812, GSE159556, GSE167747) and the analysis method of single-cell technology, the functional differences of T lymphocytes in various organs of patients after infection with SARS-CoV-2 were analyzed. Results Through single-cell data extraction of 16 livers, 19 hearts,2 spleens, 6 brains, 58 lungs, 21 kidneys and 5 pancreases from SARS-CoV-2 infected patients, invasion genes were relatively highly expressed in T lymphocytes of the lung and pancreas. The lung had a special ability to express the interferon signaling pathway, while the expression of other organs was relatively low;at the same time, the T lymphocytes of the lung also highly expressed fatty acid binding sites. Conclusion After SARS-CoV-2 infection, compared with other organs, the lung has a special interferon-activated signaling pathway and fatty acid binding site.

关 键 词：计算生物学 生物信息学 SARS-CoV-2 COVID-19 T淋巴细胞 代谢 干扰素 

分 类 号：R563.1[医药卫生—呼吸系统]