CCBE1在舌鳞癌旁组织中的表达与癌周淋巴管增生及患者预后的相关性  

High expression of CCBE1 in adjacent tissues of tongue squamous cell carcinoma is correlated with pericancerous lymphatic vessel proliferation and poor 5-year survival outcomes

作　　者：钟江龙 王洁琪[1] 叶秀华[1] 范松[1] 王友元[1] 陈伟良[1] ZHONG Jianglong;WANG Jieqi;YE Xiuhua;FAN Song;WANG Youyuan;CHEN Weiliang(Department of Oral and Maxillofacial Surgery,Sun Yat-sen Memorial Hospital,Sun Yat-sen University,Guangzhou 510120,China)

机构地区：[1]中山大学孙逸仙纪念医院口腔颌面外科,广东广州510120

出　　处：《南方医科大学学报》2022年第10期1545-1551,共7页Journal of Southern Medical University

基　　金：广东省基础与应用基础研究基金企业联合基金面上项目(2021A1515220138)。

摘　　要：目的 探究CCBE1在舌鳞癌癌旁组织中的表达与癌周淋巴管增生、颈淋巴结转移及患者预后间是否存在相关性。方法对44例cTN舌鳞癌癌旁组织病理切片标本进行淋巴管密度定量分析,采用D2-40作为淋巴管内皮标记物标定淋巴管并进行计数。将淋巴管密度低的22例纳入A组,淋巴管密度相对高的22例纳入B组。同时对两组标本中胶质和钙结合表皮生长因子域 1(CCBE1)的表达情况通过免疫组化、免疫荧光和Western blot进行测定。免疫组化检测90例舌鳞状细胞癌病理标本中CCBE1 的表达情况,并完善相关随访资料,进行统计分析。结果 免疫组化及 Western blot 结果显示 A 组中 CCBE1 的高表达率明显低于B组,差异有统计学意义(P<0.05)。免疫荧光实验显示CCBE1与D2-40存在“共定位”现象。90例舌鳞癌患者中发生颈淋巴结转移者CCBE1的高表达率较之无淋巴结转移者明显更高(P<0.05),死亡组CCBE1的高表达率也明显高于存活组(P<0.05)。结论 CCBE1在舌鳞癌组织中的表达量与癌周淋巴管增生、颈淋巴结转移及患者的5年生存率有关,可作为判断舌鳞癌患者预后的独立预测因子。Objective To examine the correlation of CCBE1 expression in adjacent tissues of tongue squamous cell carcinoma(TSCC) with pericancerous lymphatic vessel proliferation, cervical lymph node metastasis and survival outcomes of the patients. Methods Lymphatic vessel density was quantified in pericancerous tissue sections of 44 cases of cTNTSCC using D2-40 as the lymphatic vessel endothelial marker for calibration and counting of the lymphatic vessels. Of these 44 cases, 22showed a relatively low lymphatic vessel density (group A) and the other 22 had a high lymphatic vessel density (group B),and the expression levels of CCBE1 in the adjacent tissues determined using immunohistochemistry, immunofluorescence assay and Western blotting were compared between the two groups. The expression level of CCBE1 was also measured in another 90 patients with TSCC using immunohistochemistry, and all the patients were followed up for their survival outcomes.Results Immunohistochemistry and Western blotting showed a significantly lower rate of high CCBE1 expression in group A than in group B (P<0.05). Immunofluorescence assay showed co- localization of CCBE1 and D2- 40 in the adjacent tissues of TSCC. In the 90 TSCC patients with complete follow-up data, a high expression of CCBE1 was found to correlate with lymph node metastasis and a poor 5-year survival outcomes of the patients (P<0.05). Conclusion A high expression of CCBE1 in the adjacent tissues of TSCC is closely related with pericancerous lymphatic vessel proliferation, cervical lymph node metastasis and a poor 5-year survival of the patients, suggesting the value of CCBE1 as a potential prognostic predictor for TSCC.

关 键 词：舌鳞状细胞癌 胶原和钙结合EGF域1 淋巴管生成 

分 类 号：R739.8[医药卫生—肿瘤]