羧甲基多孔淀粉表征及其对槲皮素吸附研究  

The characterization of carboxymethyl porous starch and its adsorption on quercetin

作　　者：王宇霞 马云翔 苟丽娜 李敏[1] 张盛贵[1] WANG Yuxia;MA Yunxiang;GOU Lina;LI Min;ZHANG Shenggui(College of Food Science and Engineering,Gansu Agricultural University,Lanzhou 730070,China)

机构地区：[1]甘肃农业大学食品科学与工程学院,甘肃兰州730070

出　　处：《食品与发酵工业》2022年第20期105-111,共7页Food and Fermentation Industries

基　　金：甘肃省重点研发项目(20YF8NA33);中央引导地方科技发展专项(ZCYD-2020-3)。

摘　　要：以马铃薯多孔淀粉(porous starch,PS)为材料,制备具有较好溶解性和吸附性的羧甲基多孔淀粉(carboxymethyl porous starch,CMPS),并应用于生物活性物质载体。实验通过N_(2)-吸/脱附、扫描电镜、液态核磁、吸附性能分析等方法,研究了制备样品CMPS的特性。结果表明,以PS为主体,成功制备了取代度为0.25~1.44的CMPS。PS的比表面积(42.88 m^(2)/g)最大,羧甲基化后CMPS的比表面积(12.06 m^(2)/g)减小,但较羧甲基淀粉(carboxymethyl starch,CMS)的比表面积(0.87 m^(2)/g)明显增加。多孔和羧甲基处理均使淀粉结晶消失,醚化反应降低了淀粉的热稳定性。CMPS吸附槲皮素(carboxymethyl porous starch@quercetin,CMPS@Q)的负载率为13.71%,较CMS吸附槲皮素(carboxymethyl starch@quercetin,CMS@Q)负载率(0.57%)提高了95.84%。与不溶于水的槲皮素对比,CMPS@Q的水溶性(34.00%)较CMS@Q水溶性(23.33%)提高了45.74%。制备的CMPS具备良好的多孔结构、比表面积、溶解性和吸附性能,可作为低水溶性功能性物质载体,具有良好的应用前景。Potato porous starch(PS)is used as a material to prepare carboxymethyl porous starch(CMPS)with better solubility and adsorption,then CMPS will be used as a carrier of biologically active substances.The experiment used N_(2)-adsorption/desorption,scanning electron microscopy(SEM),liquid nuclear magnetic(1H NMR),adsorption performance analysis and other methods to study the characteristics of the prepared samples CMPS.The results showed that carboxymethyl porous starch(CMPS)with a degree of substitution(DS)of 0.25-1.44 was successfully prepared.The specific surface area of PS(42.88 m^(2)/g)was the largest.After carboxymethylation,the specific surface area(12.06 m^(2)/g)of CMPS decreased.However,compared with the specific surface area(0.87 m^(2)/g)of carboxymethyl starch(CMS),it was still significantly increased.Both porous and carboxymethyl treatments made the starch crystal disappear,and the etherification reaction reduced the thermal stability of the starch.The loading rate of CMPS@Q was 13.71%,which was 95.84%higher than the loading rate of CMS@Q(0.57%).Compared with quercetin insoluble in water,the water solubility of CMPS@Q(34.00%)was 45.74%higher than that of CMS@Q(23.33%).The prepared CMPS has a good porous structure,specific surface area,solubility and adsorption performance,and can be used as a carrier of low water-soluble functional substances.

关 键 词：多孔淀粉 羧甲基淀粉 比表面积 结构表征 槲皮素 

分 类 号：TS231[轻工技术与工程—粮食、油脂及植物蛋白工程]