IL-32通过PI3K/AKT/mTOR信号通路抑制胃癌细胞自噬  

IL-32 inhibits autophagy in gastric cancer cells through PI3K/AKT/mTOR signaling pathway

作　　者：王雪梅[1] 程玉[1] 齐洁敏[1] WANG Xue-mei;CHENG Yu;QI Jie-min(Department of Pathology,Chengde Medical University,Chengde 067000,China)

机构地区：[1]承德医学院病理学教研室,承德067000

出　　处：《临床与实验病理学杂志》2022年第9期1051-1056,共6页Chinese Journal of Clinical and Experimental Pathology

基　　金：承德医学院-国家自然科学基金项目培育基金(202114);承德医学院-校级重点课题基金(201711)。

摘　　要：目的探讨对人胃癌细胞自噬的影响及其可能的作用机制。方法体外培养人胃癌细胞株,应用RNA干扰技术沉默IL-32基因表达,设立阴性对照组(siNC)及实验组(siIL-32#1、siIL-32#2)。采用免疫荧光技术检测IL-32对胃癌细胞自噬的影响。Western blot法检测自噬相关蛋白LC3、p62和Beclin1以及PI3K/AKT/mTOR信号通路的关键蛋白mTOR、p-mTOR、AKT、p-AKT、p110α、p85α的表达。结果沉默IL-32基因后,胃癌细胞MGC-803和AGS的LC3荧光斑点数量显著增多(P<0.05);Western blot检测结果显示,沉默IL-32基因后,p62表达水平显著下调(P<0.05),LC3-Ⅱ和Beclin1表达水平明显上调(P<0.05);且显著降低信号通路关键蛋白p-mTOR/mTOR、p-AKT/AKT、p110α、p85α的表达(P<0.05)。结论IL-32可抑制胃癌细胞发生自噬,其作用机制可能与PI3K/AKT/mTOR信号通路有关。Purpose To investigate the effect of interleukin-32(IL-32)on autophagy in human gastric cancer cells and its possible mechanism.Method Human gastric cancer cell lines were cultured in vitro,and the expression of IL-32 gene was silenced by RNA interference.The negative control group(siNC)and the experimental group(si-IL-32#1 and si-IL-32#2)were established.The effect of IL-32 on autophagy of gastric cancer cells was detected by immunofluorescence technique.Western blot was used to detect the expression of autophagy related proteins LC3,p62 and Beclin1,as well as key proteins mTOR,p-mTOR,AKT,p-AKT,p110αand p85αin PI3K/AKT/mTOR signaling pathway.Results After silencing IL-32 gene,the number of LC3 fluorescent spots in gastric cancer MGC-803 cells and AGS cells increased significantly(P<0.05).Western blot showed that after silencing IL-32 gene,p62 expression was significantly down-regulated(P<0.05),LC3-Ⅱand Beclin1 expression were significantly up-regulated(P<0.05).The expressions of p-mTOR/mTOR,p-AKT/AKT,p110αand p85αin the signal pathway were significantly decreased(P<0.05).Conclusion IL-32 can inhibit autophagy in gastric cancer cells,and its mechanism may be related to PI3K/AKT/mTOR signaling pathway.

关 键 词：胃肿瘤 IL-32 自噬 PI3K/AKT/mTOR信号通路 

分 类 号：R735.2[医药卫生—肿瘤]