石柱单枝连“形、色、味、量”等级评价研究  

Study on the grade evaluation of appearance,color,taste and quantity of Coptidis Rhizoma from Shizhu

作　　者：冉继春 秦伟瀚[1] 谭春斌[1] 阳勇[1] 张小梅[1] 樵星芳[1] 吉光见稚代 RAN Ji-chun;QIN Wei-han;TAN Chun-bin;YANG Yong;ZHANG Xiao-mei;QIAO Xing-fang;YOSHIMITSU Michiyo(Chongqing Academy of Chinese Materia Medica,Chongqing 400065,China)

机构地区：[1]重庆市中药研究院,重庆400065

出　　处：《天然产物研究与开发》2022年第10期1755-1763,共9页Natural Product Research and Development

基　　金：重庆市自然科学基金面上项目(cstc2020jcyj-msxmX0534);重庆市绩效激励引导专项(jxjl20210004)。

摘　　要：采用色度仪、电子舌技术对石柱单枝连颜色、味道特征进行数字化表征,探讨其颜色、味道、生物碱含量、外观性状间的相关性,SPSS 22.0相关性分析显示,石柱单枝连的外观值与味道值和颜色值呈显著相关性,生物碱含量与味道、颜色之间也具有显著相关性,各味觉值之间也具有显著相关性;而颜色与味道无显著相关性。以外观、颜色、味道、生物碱含量为变量,采用k均值分类法将黄连聚为三类,并根据聚类结果划分为3个等级,通过SIMCA 14.1中的PLS-DA分析模型实现等级的有效鉴别。其中长度值是等级划分的最重要的影响因素,其次是颜色中的b值,在味道值中的药物苦味是最重要的影响因素,生物碱含量中巴马汀含量是最重要的影响因素。该研究实现石柱黄连的“色、味”数字化信息表达,填补石柱黄连的“色、味”数字化等级标准的空白,建立石柱单枝连基于“形、色、味、量”划分等级的标准模型,为中药材“色、味”数字化鉴别与等级划分奠定基础,为中药材整体性品质评价提供参考。Colorimeter and electronic tongue technologies were used to digitally characterize the color and taste characteristics of Coptidis Rhizoma,and to explore the correlation between the color,taste,alkaloid content and appearance characteristics.The correlation obtained by SPSS 22.0 showed that the appearance value of Coptidis Rhizoma was significantly correlated with the taste and color,and alkaloid content was significantly correlated with the taste and color.The taste value was significantly correlated with each other,but there was no significant correlation between the color and taste.By using appearance,color,taste and alkaloid content as variables,Coptidis Rhizoma samples were clustered into three categories via the k-means classification method,and thus divided into three grades according to the clustering results.The PLS-DA analysis model by SIMCA 14.1 was used to achieve effective classification.Length is the most important factor in the classification,followed by the b in the color value,the B-bitterness2 in the taste is the most important factor,and the plmatine content is the most important factor.This research realized the digital information expression of color and taste of Coptidis Rhizoma,and filled the blank of the digital grade standard of color and taste of Coptidis Rhizoma.We establish a standard model for the classification of Coptidis Rhizoma based on shape,color,taste and quantity,which lays a foundation for the digital identification and classification of color and taste of Chinese medicinal materials and provides a reference for the overall quality evaluation of Chinese medicinal materials.

关 键 词：黄连 电子舌 色度仪 等级 

分 类 号：R282.5[医药卫生—中药学]