MAGE-A3在非小细胞肺癌中表达与肿瘤血管生成相关性研究  

Correlation between MAGE-A3 expression and tumor angiogenesis in non-small cell lung cancer

作　　者：刘帮助 刘小霞[1] 李茜[1] 鲍姨琴 李世荣 杨刚[1] 黄礼年[2] LIU Bang-zhu;LIU Xiao-xia;LI Qian;BAO Yi-qin;LI Shi-rong;YANG Gang;HUANG Li-nian(Department of Respiratory Medicine,Wuhu Hospital affiliated to East China Normal University,Wuhu,Anhui 241000,China;Department of Respiratory and Critical Care Medicine,The First Affiliated Hospital of Bengbu Medical College,Bengbu,Anhui 233004,China)

机构地区：[1]华东师范大学附属芜湖医院呼吸内科,安徽芜湖241000 [2]蚌埠医学院第一附属医院医院呼吸危重症学科,安徽蚌埠233004

出　　处：《临床肺科杂志》2022年第11期1711-1717,共7页Journal of Clinical Pulmonary Medicine

基　　金：安徽省自然科学基金(No.1708085MH210)。

摘　　要：目的探讨MAGE-A3在非小细胞肺癌(NSCLC)患者组织中表达与肿瘤血管生成相关性,并评估其预后的价值。方法免疫组化法测145例NSCLC患者组织标本中MAGE-A3、VEGF-A与MVD共表达与血管生成关系;分析M^(+)V^(+)组,M^(+)V^(-)组,M^(-)V^(+)组,M^(-)V^(-)组的临床特征及预后;构建MAGE-A3基因RNAi慢病毒载体并转染细胞株H1299,MTT法检测细胞增殖;Transwell小室法检测细胞迁移及侵袭能力的影响。结果(1)MAGE-A3与VEGF-A、MVD在NSCLC患者中表达阳性率无统计学差异性(P>0.05);而MAGE-A3表达阳性组患者存在鳞癌表达阳性率高、肿瘤体积大、分化程度低、TNM分期晚和淋巴结转移阳性临床特征(P<0.05)。(2)MAGE-A3与VEGF-A在NSCLC中的表达水平呈正相关;MAGE-A3/VEGF-A在NSCLC患者组织中表达阳性组比阴性组的中位总生存期短,且共表达组分析均短于M-V-组。(3)在体外,下调H1299细胞株中MAGE-A3 mRNA表达后,VEGF-A与MVD蛋白表达水平、细胞的迁移、侵袭及增殖能力均显著降低(P<0.05)。结论MAGE-A3在NSCLC患者组织中表达,具有促肿瘤血管生成作用;可作为判定预后的指标。Objective To explore the correlation between the expression of MAGE-A3 in non-small cell lung cancer(NSCLC)patients and tumor angiogenesis,and to evaluate its prognostic value.Methods The relationship between the co-expression of MAGE-A3,VEGF-A,MVD,and angiogenesis in tissue samples of 145 NSCLC patients was detected by immunohistochemistry;the clinical features and prognosis of the M^(+)V^(+)group,M^(+)V^(-)group,M^(-)V^(+)group,and M^(-)V^(-)group were analyzed;The RNAi lentivirus vector of MAGE-A3 gene was constructed and infected with cell line H1299.Cell proliferation was detected by MTT assay.Transwell assay was used to detect changes in cell migration and invasion.Results 1.There was no significant difference in the positive rate of MAGE-A3,VEGF-A,and MVD in NSCLC patients(P>0.05);however,the MAGE-A3 positive group had a high positive rate of squamous cell carcinoma,large tumor volume and low degree of differentiation,late TNM stage and positive clinical features of lymph node metastasis(P<0.05).2.The expression of MAGE-A3 and VEGF-A in NSCLC were positively correlated;the MAGE-A3/VEGF-A positive group in NSCLC patient tissue had a shorter median overall survival time than the negative group,and the analysis of the co-expression group was shorter than M-V-group.3.In vitro,after down-regulating the expression of MAGE-A3 mRNA in the H1299 cell line,the expression levels of VEGF-A and MVD proteins,the cell migration,invasion,and proliferation abilities were significantly decreased(P<0.05).Conclusion MAGE-A3 is expressed in the tissues of NSCLC patients and has the effect of promoting tumor angiogenesis,which can be used as an indicator to determine the prognosis.

关 键 词：非小细胞肺癌 血管内皮生长因子 微血管密度 黑色素瘤抗原A3 

分 类 号：R734.2[医药卫生—肿瘤]