生命早期营养干预对雌性大鼠青春发育的影响  

Early-Life Nutrition Intervention Influences Pubertal Development in Female Rats

作　　者：陈丹纯 唐本玉[1] 朱顺叶[1] CHEN Dan-chun;TANG Ben-yu;ZHU Shun-ye(Department of Pediatrics,The Third Affiliated Hospital of Sun Yat-sen University,Guangzhou 510632,China)

机构地区：[1]中山大学附属第三医院儿科,广东广州510632

出　　处：《中山大学学报（医学科学版）》2022年第5期730-737,共8页Journal of Sun Yat-Sen University:Medical Sciences

基　　金：广东省科技计划项目(2014A020212392);广州市天河区科技计划项目医疗卫生专项(201404KW029)。

摘　　要：【目的】本研究旨在探讨生命早期营养干预对雌性大鼠青春期发育的影响。【方法】采用胎儿期限食法建立小于胎龄儿(SGA)雌性大鼠模型(IS组和IC组,分别代表小于胎龄儿生后营养过剩小组和小于胎龄儿生后营养正常组),测量不同营养干预方式大鼠的阴道开放时间,检测血清基础性激素水平,计算子宫和卵巢器官系数,并计算不同发育阶段大鼠的成熟黄体数量。【结果】生后营养过剩雌性大鼠(IS组和CS组,CS组代表适于胎龄儿生后营养过剩组)的阴道开放时间较正常营养的雌性大鼠(CC组和IC组,CC组代表适于胎龄儿生后营养正常组)提前;IS组和CS组生后35 d黄体生成素(LH)水平高于CC组;同时,CC组、IS组和CS组大鼠生后35 d雌二醇和LH水平高于同组生后21 d的水平。生后35 d和45 d,IS组和CS组成熟黄体数量高于CC组。各组大鼠生后35 d的子宫和卵巢器官系数高于同组生后21 d。【结论】大部分SGA雌性大鼠生后正常饮食可完成追赶性生长和正常的青春期发育;而无论是否为SGA,生后营养过剩的雌性大鼠均有性早熟倾向。【Objective】To explore the influence of early-life nutrition intervention on pubertal development in female rats.【Methods】Fifty-eight neonatal female rats born small for gestational age(SGA)by using maternal food restriction were randomly divided into postnatal over-nutrition group(Group IS,n=28)and postnatal normal diet group(Group IC,n=30).Another 58 neonatal female rats born appropriate for gestational age(AGA)were divided into postnatal over-nutri⁃tion group(Group CS,n=28)and postnatal normal diet group(Group CC,n=30).Then we observed and measured the vaginal opening(VO),sex hormone levels,organ coefficients of uterus and ovary,and numbers of corpora lutea at various stages of each group.【Results】The VO in Group IS and Group CS was accelerated than that in Group IC and Group CC.The levels of luteinizing hormone(LH)on postnatal day 35 in Group IS and Group CS were higher than that of Group CC.The levels of estradiol and LH on postnatal day 35 were higher than those on postnatal day 21 in Group IS,Group CS and Group CC.The organ coefficients of uterus and ovary on postnatal day 35 were higher than those on postnatal day 21 in each group.The numbers of corpora lutea on postnatal day 35 and day 45 were higher in Group IS and Group CS than those in Group CC.【Conclusions】Postnatal normal diet may help most SGA female rats catch up their growth and normal puber⁃tal development,while postnatal over-nutrition promotes precocious puberty in either SGA or AGA female rats.

关 键 词：营养干预 雌性大鼠 青春发育 小于胎龄儿 性早熟 

分 类 号：R153.1[医药卫生—营养与食品卫生学]