小剂量阿司匹林对大鼠钛合金种植体周围神经再生的影响  

Effect of Low-dose Aspirin on Peripheral Nerve Regeneration around Titanium Alloy Implant in Rats

作　　者：全知怎 陈盈哲 杨欣谕[1] 蔚一博[1] QUAN Zhi-zen;CHEN Ying-zhe;YANG Xin-yu(Department of Stomatology,Shanghai Changhai Hospital,Shanghai 200433)

机构地区：[1]上海长海医院口腔科,上海200433 [2]复旦大学附属眼耳鼻喉科医院,上海200031

出　　处：《医学临床研究》2022年第9期1325-1328,共4页Journal of Clinical Research

摘　　要：【目的】探讨小剂量阿司匹林对大鼠钛合金种植体周围神经再生的影响。【方法】40只8周龄SPF级、SD雄性大鼠随机分为对照组和观察组(阿司匹林组),每组20只,均于右侧股骨干骺端植入Ti-6Al-4V种植钉一枚。大鼠术后苏醒后,观察组给予阿莫西林67 mg/(kg·d)(3次/d,连续3 d)灌胃处理,并于当日给予小剂量阿司匹林1.786 mg/(kg·d)灌胃处理(连续8周)。对照组同时间给予等剂量的阿莫西林和0.9%氯化钠注射液灌胃处理。两组分别于药物干预后4周、8周各取10只大鼠种植体股骨组织,采用Van Gieson染色观察种植体周围骨组织形态学并计算骨小梁容积率(TBV)、骨结合率(BIC);采用免疫组化法分析并比较神经丝蛋白(NFP)阳性情况,并计算NFP蛋白阳性率。【结果】术后4周、8周,对照组和观察组大鼠种植体表面均有新骨形成,与对照组比较,观察组大鼠种植体周围股骨组织TBV、BIC均升高(P<0.05);大鼠种植体周围股骨组织NFP阳性神经结构分布在种植窝边缘、周围骨髓腔、骨小梁腔隙及骨膜中,给药干预后4周时,观察组、对照组种植体周围NFP阳性率分别为90.00%、60.00%,观察组NFP阳性率高于对照组(P<0.05);术后8周时,两组NFP阳性率均为80.00%,两组比较,差异无统计学意义(P>0.05)。【结论】小剂量阿司匹林可能增加大鼠钛合金种植体周围早期神经纤维数量,进而促进种植体周围神经功能再生。【Objective】To investigate the effect of low-dose aspirin on peripheral nerve regeneration surrounding titanium alloy implant in rats.【Methods】A total of 40 male SPF and SD rats with 8-week-old were randomly divided into the control group and the observation(aspirin)group,with 20 rats in each group.A Ti-6Al-4V implant nail was implanted in the epiphysis of the right femoral shaft.After the rats woke up post-operation,the aspirin group was given amoxicillin 67 mg/(kg·d)(3 times/day for 3 consecutive days),and low-dose aspirin 1.786 mg/(kg·d)was given by gavage on the same day(for 8 weeks).The control group was given the same dose of amoxicillin and 0.9%sodium chloride injection by gavage at the same time.The femoral tissues of 10 rats with implants were taken from the two groups at 4 weeks and 8 weeks after drug intervention,respectively.Van Gieson staining was used to observe the bone histomorphology around the implant and calculate the trabecular volume ratio(TBV)and bone binding rate(BIC).The positive expressions of neurofilament protein(NFP)protein were measured by immunohistochemical method,and the positive rate of NFP protein was calculated.【Results】At 4 and 8 weeks after operation,new bone was formed on the implant surface in the control group and the observation group.The levels of TBV and BIC in the femoral tissue around the implant in the observation(aspirin)group were higher than those in the control group(P<0.05).The NFP positive nerve structures in the femoral tissue around the implant were distributed on the edge of the implant nest,the surrounding bone marrow cavity,the bone trabecular space and periosteum.At 4 weeks after administration,the NFP positive rates around the implant in the observation group and the control group were 90.00%and 60.00%respectively.The NFP positive rate in the observation group was higher than that in the control group(P<0.05);At 8 weeks after operation,the positive rates of NFP of two groups were 80.00%.There was no significant difference between the two grou

关 键 词：阿司匹林 大鼠 钛 周围神经 神经再生 疾病模型 动物 

分 类 号：R-332[医药卫生]