外源注射褪黑素对小麦抵抗叶锈菌侵染的影响  

Effect of Exogenous Melatonin Injection on Resistance of Wheat to Leaf Rust

作　　者：王硕 赵港伊 史天乐 吴思凡 闫倩颖 韩胜芳[1] 王冬梅[1] WANG Shuo;ZHAO Gangyi;SHI Tianle;WU Sifan;YAN Qianying;HAN Shengfang;WANG Dongmei(State Key Laboratory of North China Crop Improvement and Regulation,Hebei Key Laboratory of Plant Physiology and Molecular Pathology,College of Life Sciences,Hebei Agricultural University,Baoding 071001,China)

机构地区：[1]华北作物改良与调控国家重点实验室,河北省植物生理与分子病理学重点实验室,河北农业大学生命科学学院,河北保定071001

出　　处：《华北农学报》2022年第5期181-186,共6页Acta Agriculturae Boreali-Sinica

基　　金：国家自然科学基金项目(31171472,31871548);河北省应用基础研究计划重点基础研究项目(12967149D);河北省高等学校科学技术研究项目(ZD2021054)。

摘　　要：为了探究褪黑素在小麦与叶锈菌互作中的作用,以小麦品种洛夫林10(简称L10)与叶锈菌生理小种260组成不亲和组合为研究对象,通过外源注射甲基紫精(甲基紫精作为一种氧化剂诱发产生超氧阴离子,可以有效增加活性氧的含量),诱导产生活性氧,利用褪黑素清除活性氧的能力确定褪黑素最佳作用浓度;然后在小麦幼苗叶片中注射褪黑素并接种叶锈菌生理小种260,通过DAB染色观察HO含量变化;通过Rohringer染色检测HR面积;通过测定小麦过氧化物酶和过氧化氢酶活性,探究外源注射褪黑素对小麦抗氧化能力的影响;通过以上研究以明确褪黑素在小麦与叶锈菌互作中的作用。结果表明:外源注射甲基紫精引起的活性氧增加,其清除最适的褪黑素注射浓度为10μmol/L。对不亲和组合的DAB染色结果显示,注射10μmol/L褪黑素后,叶锈菌侵染诱发的小麦叶片HO积累量少于对照组,表明褪黑素参与了HO清除作用;Rohringer染色结果表明,经过外源性褪黑素处理后小麦HR细胞面积减小,有效增强了对叶锈菌的抗性。此外,外源注射褪黑素提高了抗氧化酶POD和CAT的活性,表明小麦的抗氧化能力获得显著提升。试验结果表明,外源注射褪黑素参与了小麦抗叶锈菌过程中活性氧的清除,提高了小麦的抗病能力。In order to explore the role of melatonin in the interaction between wheat and leaf rust,we studied the incompatible combination between wheat variety Lovrin 10(hereinafter referred to as L10)and physiological race 260 of leaf rust.In this experiment,reactive oxygen species were induced by exogenous injection of Methyl viologen(Methy lviologen(MV)as an oxidant induces the production of superoxide anion,which can effectively increase the content of reactive oxygen species),and the best concentration of melatonin was determined by using the ability of melatonin to scavenge reactive oxygen species;then,melatonin was injected into the leaves of wheat seedlings and inoculated with leaf rust race 260,and the changes of HOcontent were observed by DAB staining.The HR area was detected by Rohringer staining.The effects of exogenous melatonin injection on the antioxidant capacity of wheat were investigated by measuring the peroxidase(POD)and catalase(CAT)activities of wheat;through the above research,the role of melatonin in the interaction between wheat and leaf rust was clarified.The results showed that the reactive oxygen species was increased by exogenous injection of methyl viologen,and the optimum injection concentration of melatonin was 10μmol/L.The results of DAB staining of incompatible combinations showed that after injection of 10μmol/L melatonin,the accumulation of HOinduced by leaf rust infection in wheat leaves was less than that in control group,which indicated that melatonin participated in HOscavenging.Rohringer staining showed that the area of wheat HR cells decreased after exogenous melatonin treatment,which effectively enhanced the resistance to leaf rust.In addition,exogenous injection of melatonin increased the activities of antioxidant enzymes POD and CAT,which indicated that the antioxidant capacity of wheat was significantly improved.The results showed that exogenous injection of melatonin was involved in the removal of reactive oxygen species in the process of wheat resistance to leaf rust,and i

关 键 词：小麦 褪黑素 活性氧 DAB HR 抗氧化酶 

分 类 号：S435.121[农业科学—农业昆虫与害虫防治] S184[农业科学—植物保护]