旋蒴苣苔CDPK基因家族鉴定与特征分析  

Identification and Characteristic Analysis of CDPK Gene Family in Boea hygrometrica

作　　者：石天龙 李欲柯 洪鲲[1] 龚纪熠 李菲[1,2] Shi Tianlong;Li Yuke;Hong Kun;Gong Jiyi;Li Fei(Key Laboratory of Plant Physiology and Development Regulation,School of Life Science,Guizhou Normal University,Guiyang,550025;Key Laboratory of State Forestry Administration on Biodiversity Conservation in Karst Mountain Area of Southwest of China,Guiyang,550025)

机构地区：[1]贵州师范大学生命科学学院,植物生理与发育调控重点实验室,贵阳550025 [2]国家林业和草原局西南喀斯特山地生物多样性保护重点实验室,贵阳550025

出　　处：《分子植物育种》2022年第18期6015-6023,共9页Molecular Plant Breeding

基　　金：国家自然科学基金委员会-贵州省人民政府喀斯特科学研究中心项目(U1812401)资助。

摘　　要：钙依赖蛋白激酶(CDPKs)是植物特有的一类丝氨酸/苏氨酸型蛋白激酶。CDPK在介导植物生长发育和逆境信号转导中具有重要作用。大多数高等植物无法耐受极度脱水状态,然而旋蒴苣苔进化出独特的耐脱水机制,能够耐受体内水分丧失90%以上,且复水后迅速恢复生活状态。为揭示旋蒴苣苔CDPK基因在生长发育与抗旱防御机制中的作用,本研究基于旋蒴苣苔基因组数据,在全基因组水平利用生物信息学工具对CDPK基因家族成员进行鉴定,并对其系统进化、基因结构、染色体定位等进行分析并对部分基因进行功能预测。本研究鉴定了旋蒴苣苔CDPK基因家族,并对其进行相关分析,为在基因组范围内研究CDPK基因在旋蒴苣苔抗旱逆境响应中的功能提供了理论基础。Calcium-dependent protein kinases(CDPKs)are a class of serine/threonine-type protein kinases unique to plants,and CDPKs play an important role in mediating plant growth and development and signaling in response to adversity.Most higher plants are unable to tolerate extreme dehydration,however,Boea hygrometric has evolved a unique dehydration tolerance mechanism,able to tolerate more than 90%loss of body water and rapidly resume living conditions after rehydration.In order to reveal the role of CDPK genes in the growth and development and drought defense mechanism,this study used bioinformatics tools to identify CDPK gene family members at the genome-wide level,analyze their phylogeny,gene structure,chromosomal localization,and predict the functions of some genes based on the genomic data of Boea hygrometric.The CDPK gene family identified in this study and its correlation analysis provide a theoretical basis for a genome-wide study of the function of CDPK genes in the drought stress response of Boea hygrometrica.

关 键 词：旋蒴苣苔 CDPK 生物信息学 

分 类 号：Q943.2[生物学—植物学]