VEGF促进内皮祖细胞参与大鼠正畸实验模型牙周组织改建的研究  

VEGF promotes the involvement of endothelial progenitor cells in periodontal tissue remodeling in rat orthodontic experimental model

作　　者：印倩文 谷德奥 熊丽平 苗雷英[2] 刘超[1] YIN Qianwen;GU Deao;XIONG Liping;MIAO Leiying;LIU Chao(Department of Orthodontics,Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210008,China;Department of Cariology and Endodontics,Nanjing Stomatological Hospital,Medical School of Nanjing University,Nanjing 210008,China)

机构地区：[1]南京大学医学院附属口腔医院,南京市口腔医院口腔正畸科,江苏南京210008 [2]南京大学医学院附属口腔医院,南京市口腔医院牙体牙髓科,江苏南京210008

出　　处：《口腔生物医学》2022年第3期148-153,共6页Oral Biomedicine

基　　金：国家自然科学基金(51972167);南京市卫生厅医学科技发展基金资助一般项目(YKK18126)。

摘　　要：目的:观察血管内皮祖细胞(EPC)在正畸实验大鼠牙周组织改建区的分布,探讨血管内皮细胞生长因子(VEGF)对其趋化的影响。方法:建立实验性大鼠牙移动模型,分离、培养并鉴定大鼠外周血EPC。在体外实验中以VEGF干预EPC,观察其增殖、迁移能力。将BrdU标记的EPC通过尾静脉注入模型大鼠体内,观察EPC在牙周组织中的分布情况及VEGF的表达。结果:分离培养的大鼠EPC,在牙周移动大鼠外周血富集,完成细胞表型鉴定。VEGF可促进EPC体外增殖能力(P<0.05),对EPC的趋化、迁移有促进作用。经BrdU标记的EPC注入模型大鼠体内后,牙周组织BrdU EPC阳性数目增加;VEGF在实验组(尾静脉注射EPC细胞)的张力侧的表达高于对照组(注射生理盐水)。结论:正畸牙移动时外周血EPC可趋化聚集于牙周组织,并在VEGF的调控下参与牙周组织改建。Objective:To observe the distribution of vascular endothelial progenitor cell(EPC)in the periodontal tissue remodeling area of orthodontic experimental rats and to investigate the effect of vascular endothelial growth factor(VEGF)on their chemotaxis and differentiation.Methods:Experimental rat tooth movement model was established,and rat peripheral blood EPC were isolated,cultured and identified.The proliferation and migration of EPC were observed by intervening VEGF in vitro.BrdU-labeled EPC were injected into model rats through the tail vein to observe the distribution of EPC in periodontal tissues,and the expression of VEGF.Results:Cell phenotype identification was completed by isolating and culturing rat EPC.VEGF can promote the proliferation ability of EPC in vitro and promote the chemotaxis and migration of EPC.After injection of BrdU-labeled EPC into model rats,the number of BrdU EPC positive periodontal tissues increased;the expression of VEGF on the tension side of the experimental group(tail vein injection of EPC cells)was higher than that of the control group(injection of saline).Conclusions:During orthodontic tooth movement,peripheral blood EPC can be chemotactic and accumulate in periodontal tissue,and participate in periodontal tissue remodeling under the regulation of VEGF.

关 键 词：正畸牙移动 血管内皮祖细胞 血管生成 血管内皮细胞生长因子 

分 类 号：R783.5[医药卫生—口腔医学]