二茂铁维甲酸纳米粒子诱导口腔鳞状细胞癌细胞发生铁死亡  

Ferroptosis of oral squamous cell carcinoma induced by ferrocenyl retinoic acid nanoparticles

作　　者：邢如霄 孙宾[2] XING Ruxiao;SUN Bin(Department of Stomatology,Shijiazhuang Second Hospital,Shijiazhuang 050000)

机构地区：[1]石家庄市第二医院口腔科,河北石家庄050000 [2]吉林大学口腔医学院口腔颌面外科,吉林长春130021

出　　处：《口腔生物医学》2022年第3期165-168,174,共5页Oral Biomedicine

基　　金：吉林省科技发展计划项目(20200403119SF);吉林省教育厅科学技术研究项目(JJKH20211219KJ)。

摘　　要：目的:研究二茂铁维甲酸纳米粒子(FCRA Nps)诱导口腔鳞状细胞癌(OSCC)细胞发生铁死亡的作用。方法:培养人OSCC细胞CAL27,利用CCK-8法测定FCRA Nps对CAL27细胞增殖作用的影响;荧光显微镜观察FCRA Nps对CAL27细胞内谷胱甘肽(GSH)、活性氧(ROS)含量的影响;采用线粒体红色荧光探针特异性地标记细胞内具有生物活性的线粒体,Hoechst 33342染色液标记细胞核,荧光显微镜下观察FCRA Nps对CAL27细胞线粒体及细胞核形态的影响。结果:与对照组比较,FCRA Nps对CAL27细胞增殖具有抑制作用;FCRA Nps作用使得CAL27细胞内GSH含量减少,ROS含量增多(P<0.01);与对照组相比,FCRA Nps组线粒体体积变小,细胞核无明显变化。结论:FCRA Nps可诱导OSCC细胞发生铁死亡。Objective:To study the effect of ferrocenyl retinoic acid nanoparticles(FCRA Nps)on ferroptosis induced by oral squamous cell carcinoma cells(OSCC).Methods:Human OSCC cells CAL27 were cultured,and the effect of FCRA Nps on the proliferation of CAL27 cells was determined by CCK-8 method;the effects of FCRA Nps on the contents of glutathione(GSH)and reactive oxygen species(ROS)in CAL27 cells were observed by fluorescence microscope;Mitochondrial red fluorescent probe was used to specifically label the biologically active mitochondria in cells,Hoechst 33342 staining solution was used to label the nucleus,and the effect of FCRA Nps on the morphology of mitochondria and nucleus in CAL27 cells was observed under a fluorescence microscope.Results:Compared with the control group,FCRA Nps had an inhibitory effect on the proliferation of CAL27 cells;the effect of FCRA Nps reduced the content of GSH and increased the content of ROS in CAL27 cells(P<0.01);compared with the control group,the mitochondrial volume of the FCRA Nps group became smaller,the nucleus did not change significantly.Conclusions:FCRA Nps can induce ferroptosis in OSCC cells.

关 键 词：二茂铁维甲酸 口腔鳞癌细胞 铁死亡 纳米粒子 

分 类 号：R739.85[医药卫生—肿瘤]