基于RANKL/OPG通路探讨GsMTx4对大鼠正畸牙齿移动过程中压力侧牙周组织改建的影响  

Effect of GsMTx4 on periodontal tissue reconstruction at the pressure side during orthodontic tooth movement in rats through RANKL/OPG pathway

作　　者：王贺 李易 张曼 张严匀 张苗苗[1] WANG He;LI Yi;ZHANG Man;ZHANG Yanyun;ZHANG Miaomiao(Department of Orthodontics,School of Stomatology,Harbin Medical University,Harbin 150001,China)

机构地区：[1]哈尔滨医科大学口腔医学院口腔正畸科,黑龙江哈尔滨150001

出　　处：《口腔生物医学》2022年第3期169-174,共6页Oral Biomedicine

摘　　要：目的:从核因子-κB受体活化因子配体(RANKL)/骨保护素(OPG)通路探讨Piezo1蛋白通道的抑制剂GsMTx4对大鼠正畸牙齿移动过程中压力侧牙周组织改建的影响。方法:75只健康SD雄性大鼠构建正畸模型,随机分为对照、加力、加力+GsMTx4组。测量第一磨牙近中移动距离,HE染色观察上颌第一磨牙近中根压力侧牙周组织病理变化,免疫组化染色观察RANKL、OPG在压力侧牙周组织的表达量及定位,并观察RANKL/OPG比值的变化,抗酒石酸酸性磷酸酶染色观察压力侧牙周组织中破骨细胞数量变化。结果:随着加力时间的延长,牙齿移动距离逐渐增加,加力组牙齿移动距离高于加力+GsMTx4组(P<0.05);加力组与加力+GsMTx4组RANKL、RANKL/OPG比值以及破骨细胞数量均呈现先升高后降低的趋势,OPG呈现逐渐上升的趋势,且加力+GsMTx4组的值低于加力组(P<0.05)。结论:GsMTx4能够降低大鼠正畸牙齿移动过程中压力侧牙周组织中RANKL、OPG蛋白的表达,并且能够抑制破骨细胞的分化,从而降低骨吸收活动,进而抑制牙齿移动。Objective:To explore the effect of the inhibitor GsMTx4 of Piezo1 protein channel on periodontal tissue reconstruction at the pressure side during orthodontic tooth movement in rats through RANKL/OPG pathway.Methods:The orthodontic model was established in 75 healthy male SD rats.The rats were randomly divided into control group,force group and force+GsMTx4 group.The mesial movement distance of the first molars was measured.HE staining was used to observe the pathological changes of periodontal tissue in the pressure side of the proximal root of the mandibular first molars.Immunohistochemical staining was used to observe the expression and localization of nuclear factor-κB receptor activator ligand(RANKL)and OPG in the periodontal tissue at pressure side.The change of the ratio of RANKL to osteoprotegerin(OPG)and the number of osteoclasts in periodontal tissues at the pressure side through tartrate-resistant acid phosphatase staining were observed.Results:With the extension of force time,the tooth movement distance gradually increased,and tooth movement distance in force group was higher than that in force+GsMTx4 group(P<0.05).RANKL,the ratio of RANKL/OPG and the number of osteoclasts in force group and force+GsMTx4 group showed a trend of increasing first and then decreasing,and OPG showed a gradual increasing trend.Compared with force group,the values in force+GsMTx4 group were all decreased(P<0.05).Conclusions:GsMTx4 can reduce the expression of RANKL and OPG proteins in the periodontal tissue at the pressure side during orthodontic tooth movement in rats,and inhibit osteoclasts differentiation,thereby reducing bone resorption activity and inhibiting tooth movement.

关 键 词：GsMTx4 正畸牙齿移动 RANKL OPG Piezo1 破骨细胞 

分 类 号：R783.5[医药卫生—口腔医学]