土槿皮乙酸通过miR-4282抑制人弥漫大B细胞淋巴瘤细胞系SU-DHL-4的增殖  

Pseudolaric acid B inhibits the proliferation of human diffuse large B-cell lymphoma cell line SU-DHL-4 via miR-4282

作　　者：袁军[1] 韩虎 董巍[3] 王瑞仓[1] 郝洪岭[1] 张学军[4] YUAN Jun;HAN Hu;DONG Wei;WANG Rui-cang;HAO Hong-ling;ZHANG Xue-jun(Department of Hematology,Hebei Provincial People's Hospital,Shijiazhuang 050055;Department of Emergency,Hebei Provincial People's Hospital,Shijiazhuang 050055;Department of Cardiology,Shijiazhuang Third Hospital,Shijiazhuang 050000;Department of Hematology,the Second Hospital of Hebei Medical University,Shijiazhuang 050055,China)

机构地区：[1]河北省人民医院血液科,河北石家庄050055 [2]河北省人民医院急诊科,河北石家庄050055 [3]石家庄市第三医院心内科,河北石家庄050000 [4]河北医科大学第二医院血液内科,河北石家庄050055

出　　处：《基础医学与临床》2022年第11期1731-1736,共6页Basic and Clinical Medicine

基　　金：河北省医学科学研究课题计划(20190282)。

摘　　要：目的观察土槿皮乙酸(PAB)通过调控miR-4282对人弥漫大B细胞淋巴瘤细胞系SU-DHL-4增殖和凋亡的影响。方法将SU-DHL-4细胞分为对照组、PAB-L组、PAB-M组、PAB-H组。按照脂质体法将miR-NC、miR-4282转染至细胞,记为miR-NC组、miR-4282组;将anti-miR-NC、anti-miR-4282转染至细胞,用20μmol/L PAB培养细胞,记为PAB+anti-miR-NC组、PAB+anti-miR-4282组。采用CCK8法检测细胞增殖;流式细胞测量术检测细胞凋亡;Western blot检测Ki-67、cleaved caspase-3、cleaved caspase-9蛋白表达;RT-qPCR检测miR-4282表达。结果与对照组相比,PAB-L组、PAB-M组、PAB-H组Ki-67蛋白表达降低,miR-4282表达量及凋亡率、细胞增殖抑制率、蛋白cleaved caspase-3、cleaved caspase-9表达增加(P<0.05)。与miR-NC组相比,miR-4282组Ki-67蛋白表达降低,miR-4282表达量及凋亡率、细胞增殖抑制率、cleaved caspase-3、cleaved caspase-9表达增加(P<0.05)。抑制miR-4282表达可以降低PAB对SU-DHL-4细胞增殖和凋亡的影响。结论土槿皮乙酸可能通过增加miR-4282抑制SU-DHL-4细胞增殖和促进凋亡。Objective To observe the effect of pseudolaric acid B(PAB)on the proliferation and apoptosis of human diffuse large B-cell lymphoma cell line SU-DHL-4 possibly and to explore the potential mechanism through regulating miR-4282.Methods SU-DHL-4 cells were divided into control group,PAB-L group,PAB-M group,PAB-H group;According to liposome method,miR-NC and miR-4282 were transfected into the cells and were marked as miR-NC group,miR-4282 group;The anti-miR-NC,anti-miR-4282 were transfected into the cells,cultured with 20μmol/L PAB,denoted as(PAB+anti-miR-NC)group and(PAB+anti-miR-4282)group.Cell pro-liferation was detected by CCK8 assay,and cell apoptosis was detected by flow cytometry.The expression of Ki-67,cleaved caspase-3,cleaved caspase-9 protein was detected by Western blot and miR-4282 expression was detected by RT-qPCR.Results Compared with the control group,Ki-67 protein expression in PAB-L group,PAB-M group,and PAB-H group was decreased while miR-4282 expression,and the apoptosis rate,cell inhibition rate,protein expression of cleaved caspase-3,cleaved caspase-9 were increased(P<0.05).Compared with the miR-NC group,Ki-67 protein expression in miR-4282 group decreased,while miR-4282 expression,the apoptosis rate,cell inhibition rate,protein expression of cleaved caspase-3,cleaved caspase-9 were all increased(P<0.05).Inhibition of miR-4282 expression reduced the effect of PAB on the proliferation and apoptosis of SU-DHL-4 cells.Conclusions PAB may inhibit the proliferation of human diffuse large B-cell lymphoma cell SU-DHL-4 and promote apoptosis through increased expression of miR-4282.

关 键 词：土槿皮乙酸 miR-4282 弥漫大B细胞淋巴瘤 增殖 凋亡 

分 类 号：R733.1[医药卫生—肿瘤]