乔松素抑制人胃癌细胞系AGS增殖、迁移和侵袭  

Pinocembrin inhibits the proliferation,migration and invasion of gastric cancer cell line AGS

作　　者：李洵[1] 付晓霞[1] 樊丽伟 汪景坤 张东姣 赵炎焕 张磊[1] 赵玉红 LI Xun;FU Xiao-xia;FAN Li-wei;WANG Jing-kun;ZHANG Dong-jiao;ZHAO Yan-huan;ZHANG Lei;ZHAO Yu-hong(Department of Gastroenterology,the 82nd Army Hospital of the Chinese People's Liberation Army,Baoding 071000,China)

机构地区：[1]中国人民解放军陆军第八十二集团军医院消化内科,河北保定071000

出　　处：《基础医学与临床》2022年第11期1744-1750,共7页Basic and Clinical Medicine

基　　金：保定市科技计划(18ZF181)。

摘　　要：目的探讨乔松素(pinocembrin)对人胃癌细胞系AGS增殖、迁移和侵袭的作用及机制。方法将AGS细胞分为不同浓度乔松素组(50、100、200和400μmol/L)、NC组、200μmol/L pinocembrin+anti-miR-NC组、200μmol/L pinocembrin+anti-miR-34a-5p组;采用CCK-8法及流式细胞测量术检测AGS细胞增殖及凋亡;采用Transwell小室法及蛋白质免疫印迹法检测AGS细胞增殖及凋亡;实时荧光定量PCR(RT-qPCR)检测miR-34a-5p表达水平。结果不同浓度乔松素降低AGS细胞的增殖活性(P<0.05),且G/G期细胞所占比例升高,S期细胞所占比例降低,MMP2、MMP9、p-PI3K、p-AKT蛋白的表达降低,细胞迁移和侵袭数量减少,miR-34a-5p的表达水平升高(P<0.05)。抑制miR-34a-5p表达减轻乔松素对AGS细胞增殖,迁移和侵袭的抑制作用。结论乔松素可通过调控miR-34a-5p抑制AGS细胞增殖、迁移和侵袭。Objective To explore the effect and mechanism of pinocembrin on the proliferation,migration and invasion of gastric cancer cell line AGS.Methods AGS cells were divided into different test groups according to concentrations of pinocembrin(50,100,200,400μmol/L),NC group,200μmol/L pinocembrin+anti-miR-NC group,200μmol/L pinocembrin+anti-miR-34 a-5 p group.The proliferation,apoptosis,migration,invasion ability and protein expression of AGS cells were detected by CCK-8 assay,flow cytometry,Transwell chamber assay and Western blot.The expression of miR-34 a-5 p was detected by real-time fluorescent quantitative PCR(RT-qPCR).Results Different concentrations of pinocembrin reduced the proliferation activity of AGS cells(P<0.05)and the proportion of in G/Gphase cells was increased while the proportion of S phase cells decreased.The expressions of MMP2,MMP9,p-PI3 K and p-AKT protein decreased.The cell migration and invasion were inhibited.The expression of miR-34 a-5 p was increased(P<0.05),which reduced the inhibitory effect of pinocembrin on the prolifera-tion,migration and invasion of AGS cells.Conclusions Pinocembrin can inhibit the proliferation,migration and invasion of AGS cells by improving the expression of miR-34 a-5 p.

关 键 词：乔松素 miR-34a-5p 增殖 迁移 侵袭 

分 类 号：R735.2[医药卫生—肿瘤]