Grazing greatly reduces the temporal stability of soil cellulolytic fungal community in a steppe on the Tibetan Plateau  

作　　者：Fei Wang Weidong Kong Mukan Ji Kang Zhao Hao Chen Linyan Yue Xiaobin Dong 

机构地区：[1]Key Laboratory of Alpine Ecology,Institute of Tibetan Plateau Research,Chinese Academy of Sciences(CAS),Beijing 100101,China [2]College of Resources and Environment,University of Chinese Academy of Sciences,Beijing 100039,China [3]CAS Center for Excellence in Tibetan Plateau Earth Sciences,Chinese Academy of Sciences,Beijing 100101,China [4]School of Life Science,Shanxi Normal University,Linfen 041004,China [5]State Key Laboratory of Earth Surface Processes and Resource Ecology,College of Resources Science and Technology,Beijing Normal University,Beijing 100875,China

出　　处：《Journal of Environmental Sciences》2022年第11期48-57,共10页环境科学学报（英文版）

基　　金：supported by the Natural National Science Foundation of China (No. 41771303);Chinese Academy of Sciences (Nos. QYZDB-SSW-DQC033, XDB15010203 and XDA19070304)。

摘　　要：Excessive livestock grazing degrades grasslands ecosystem stability and sustainability by reducing soil organic matter and plant productivity. However, the effects of grazing on soil cellulolytic fungi, an important indicator of the degradation process for soil organic matter,remain less well understood. Using T-RFLP and sequencing methods, we investigated the effects of grazing on the temporal changes of cellulolytic fungal abundance and community structure in dry steppe soils during the growing months from May to September, on the Tibetan Plateau using T-RFLP and sequencing methods. The results demonstrated that the abundance of soil cellulolytic fungi under grazing treatment changed significantly from month to month, and was positively correlated with dissolved organic carbon(DOC) and soil temperature, but negatively correlated with soil p H. Contrastingly, cellulolytic fungal abundance did not change within the fencing treatment(ungrazed conditions). Cellulolytic fungal community structure changed significantly in the growing months in grazed soils,but did not change in fenced soils. Grazing played a key role in determining the community structure of soil cellulolytic fungi by explaining 8.1% of the variation, while p H and DOC explained 4.1% and 4.0%, respectively. Phylogenetically, the cellulolytic fungi were primarily affiliated with Ascomycota(69.65% in relative abundance) and Basidiomycota(30.35%).Therefore, grazing substantially reduced the stability of soil cellulolytic fungal abundance and community structure, as compared with the fencing treatment. Our finding provides a new insight into the responses of organic matter-decomposing microbes for grassland managements.

关 键 词：Cellulolytic fungal community CBHI Stability GRAZING Dryland soil Tibetan Plateau 

分 类 号：S812.2[农业科学—草业科学]