青枯菌噬菌体RPZH6株系对烟草青枯病的生防效果及全基因组测序分析  

Control Effect of Ralstonia Phage RPZH6 Strain on Tobacco Bacterial Wilt and Its Complete Genome Analysis

作　　者：林志坚 陈长江[2] 周挺[3] 顾钢[3] 胡方平[1] 李春英[3] 蔡学清[1] LIN Zhijian;CHEN Changjiang;ZHOU Ting;GU Gang;HU Fangping;LI Chunying;CAI Xueqing(College of Plant Protection,Fujian Agriculture and Forestry University,Fuzhou 350002,China;College of Computer and Information Sciences,Fujian Agriculture and Forestry University,Fuzhou 350002,China;Institute of Tobacco Science,Fujian Provincial Tobacco Company,Fuzhou 350003,China)

机构地区：[1]福建农林大学植物保护学院,福州350002 [2]福建农林大学计算机与信息学院,福州350002 [3]福建省烟草公司烟草科学研究所,福州350003

出　　处：《中国农业科技导报》2022年第10期133-142,共10页Journal of Agricultural Science and Technology

基　　金：国家重点研发计划项目(2017YFD0201106-02);福建省引导性项目(2022N0004);中国烟草总公司福建省公司科技计划项目(2022350000240061,201735000027130)。

摘　　要：作物青枯病是世界范围的土传维管束细菌性病害,为害重、防治难。以烟草青枯菌TBRS12为宿主菌,采用双层平板法从烟草根际土壤中分离获得一株裂解性噬菌体RPZH6。采用室内盆栽法测定噬菌体RPZH6的生防效果,并通过全基因组测序、比较基因组及系统发育树进行鉴定分析。结果表明,接种后21 d,噬菌体RPZH6对烟草青枯病的防治效果为60.98%;接种后35 d,防治效果为53.85%,显著高于化学药剂氢氧化铜处理。核酸鉴定及全基因组分析显示,噬菌体RPZH6的基因组为双链DNA,全长64657 bp,GC含量为64.84%,含有92个开放阅读框,1个tRNA,其中47个基因的编码产物为注释功能蛋白,其余为功能未知的假定蛋白。比较基因组及系统发育树分析显示,噬菌体RPZH6是一株新的短尾噬菌体科、Bcep22家族成员。上述结果为该噬菌体的进一步开发应用及生防机制探讨提供了理论基础。Crops bacterial wilt is a bacterial soil-borne and vascular bundle disease in the world which is severely harmful and difficult to control. Choosing the Ralstonia solanacearum TBRS12 as host bacterium,bacteriophage RPZH6 was isolated from tobacco rhizosphere soil using double-layer plate method. The pot experiment was used to identify its biological control effect,and whole genome sequencing,comparative genome and phylogenetic tree were used to analyze the genomic structural characteristics and evolutionary relationship. The results showed that the control effect of phage RPZH6 on tobacco bacterial wilt could reach 60.98% at 21 d after inoculation,and the control effect was also 53.85% at 35 d after inoculation,which was significantly higher than that with chemical treatment of CuOH. Nucleic acid identification and whole genome analysis showed that the genome of phage RPZH6 was doublestranded DNA with a total length of 64 657 bp and GC content of 64.84%. It contained 92 open reading frames with 1 tRNA,and the coded products of 47 genes were predicted to be functional and others were putative unknown protein.Comparative genome and phylogenetic analysis showed that phage RPZH6 was a novel member of the Bcep22-like subfamily of the family Podoviridae. In conclusion,this paper cleared the biological control effect,genomic structural characteristics and evolutionary relationship of the bacteriophage RPZH6,and the results provided theoretical basis for the further application and analyzing control mechanism.

关 键 词：噬菌体 青枯雷尔氏菌 基因组分析 生物防治 

分 类 号：S476[农业科学—农业昆虫与害虫防治]