肝脏VX2原位移植瘤术后模型的建立及超声影像学评估  

Establishment of postoperative model of VX2 orthotopic liver tumor and assessment by ultrasound

作　　者：陈凯玲 张炜彬 毛丽娟 曹佳颖 韩红 毛枫 王文平 CHEN Kailing;ZHANG Weibin;MAO Lijuan;CAO Jiaying;HAN Hong;MAO Feng;WANG Wenping(Department of Ultrasound,Zhongshan Hospital,Fudan University,Shanghai 200032,China;Shanghai Institute of Medical Imaging,Shanghai 200032,China)

机构地区：[1]复旦大学附属中山医院超声科,上海200032 [2]上海市影像医学研究所,上海200032

出　　处：《肿瘤影像学》2022年第5期547-553,共7页Oncoradiology

基　　金：上海市自然科学基金(19ZR1450700,20ZR1452800);上海市临床重点专科项目(shslczdzk03501);上海申康中心重大临床研究项目(SHDC2020CR1031B)。

摘　　要：目的:构建兔VX2肝脏原位移植瘤术后模型并进行超声检查及病理学评估。方法:用15只新西兰大白兔建立肝脏VX2原位移植瘤并随机分为3组,构建肝癌术后复发模型:R0切除组,以叶间切迹为标准切除部分肝左叶,切口旁内隧道法植入VX2瘤块;R1切除组,以瘤体边缘为界行肝左叶瘤体切除术;R2切除组,切除约1/2肿瘤组织。术后第7、14、21天行超声造影(contrast-enhanced ultrasound,CEUS)检查,实验结束后处死荷瘤兔,观察大体标本的情况并取材进行病理学检查。结果:R2切除组肿瘤大小大于R0切除组和R1切除组,而且肝内多发肿瘤(100%,5/5)、肺转移(100%,5/5)及肝门处淋巴结转移(80%,4/5)的比例高于R0切除组和R1切除组。常规超声显示肝内复发VX2肿瘤呈低回声,肿瘤内部回声不均匀与时间呈显著相关(r=-0.518)。注射造影剂后,第7天42.9%(6/14)的VX2肿瘤呈整体增强;第14天及第21天,分别有64.3%(9/14)和28.6%(4/14)的肿瘤呈整体不均匀增强,35.7%(5/14)和71.4%(10/14)呈环状增强(P=0.000),增强方式与时间有显著相关性(r=0.632)。结论:本研究建立的兔VX2肝癌术后模型可为后期研究提供可靠的动物荷瘤载体。Objective:To establish postoperative recurrence model of orthotopic liver tumor in rabbit with VX2 tissue.Methods:Fifteen New Zealand rabbits were used for VX2 orthotopic liver tumors and then randomly divided into three groups to establish the postoperative model.Group R0:the interlobular incisions were used as the standard for partial left lobotomy,and the VX2 tumor were implanted in the same liver lobe by the internal tunnel method near the incision.Group R1:resection of the tumor margin as the boundary.Group R2:about 1/2 of the tumor tissues were removed.On the 7th,14th,21st day after orthotopic transplantation of VX2 tumor,contrast-enhanced ultrasound(CEUS)was performed to observe the imaging characteristics of the tumor.The rabbits were sacrificed after the experiment,the gross specimens were observed and pathological examination was conducted.Results:The tumors in Group R2 were larger than those in Group R0 and R1,and the proportion of multiple tumors in the liver(100%,5/5),lung metastasis(100%,5/5)and hilar lymph node metastasis(80%,4/5)was higher than that in Group R0 and R1.The recurrent VX2 tumor in the liver showed hypoechoic on conventional ultrasound,and the internal echogenicity was significantly correlated with time(r=-0.518).On CEUS,42.9%(6/14)VX2 tumor showed overall enhancement on 7th,14th and 21st,64.3%(9/14)and 28.6%(4/14)showed overall heterogeneous enhancement,while 35.7%(5/14)and 71.4%(10/14)showed rim-like enhancement(P=0.000),with significant correlation between enhancement mode and time(r=0.632).Conclusion:In this study,VX2 postoperative liver tumor recurrence model in rabbit was established to provide reliable animal carrier for further research.

关 键 词：肝癌 VX2肿瘤 动物模型 超声造影 经导管动脉化疗栓塞 

分 类 号：R735.7[医药卫生—肿瘤] R445.1[医药卫生—临床医学]