919糖浆对非酒精性脂肪肝大鼠肝功能保护效应及其机制研究  

Protective effect and mechanism of 919 syrup on liver function in rats with non-alcoholic fatty liver disease

作　　者：潘丹青 徐月妹[1] PAN Dan-qing;XU Yue-mei(Shanghai University of Traditionnal Chinese Medicine,Shanghai 201203;Department of Traditional Chinese Medicine,Jinshan Hospital of Fudan University,Shanghai 201508,China)

机构地区：[1]上海中医药大学,上海201203 [2]复旦大学附属金山医院中医内科,上海201508

出　　处：《川北医学院学报》2022年第10期1245-1248,共4页Journal of North Sichuan Medical College

摘　　要：目的:探讨919糖浆对非酒精性脂肪肝(NAFLD)大鼠肝功能的保护效应及其机制。方法:高脂饲料喂养复制NAFLD大鼠模型。将SD大鼠分为正常组、模型组、高剂量组、低剂量组,每组各8只。正常组大鼠不予药物干预;模型组大鼠给予生理盐水10 mL·kg^(-1)·d^(-1)进行灌胃给药;高剂量组大鼠给予919糖浆27 g·kg^(-1)·d^(-1)进行灌胃给药;低剂量组大鼠给予919糖浆6.75 g·kg^(-1)·d^(-1)进行灌胃给药。各组均干预8周,而后采用酶联免疫吸附法检测大鼠血清甘油三酯(TG)、总胆固醇(TC)、谷丙转氨酶(ALT)、谷草转氨酶(AST)、肝组织超氧化物歧化酶(SOD)及丙二醛(MDA)水平;HE染色光镜下观察肝组织形态,比较各组间差异。结果:与正常组比较,模型组大鼠肝功能TG、TC、ALT、AST含量上升,SOD降低,差异具有统计学差异(P<0.01);与模型组比较,高剂量组和低剂量组TG、TC、ALT、AST、MDA降低,SOD含量增加,差异均具有统计学意义(P<0.05);高、低剂量组相比,TG、TC、ALT水平差异均具有统计学意义(P<0.05);HE染色结果显示,模型组肝细胞细胞结构紊乱,肿胀,呈炎性浸润,高剂量组和低剂量组肝细胞病理状态有改善。结论:919糖浆可保护NAFLD大鼠肝功能,减轻肝细胞损伤,其机制可能与增强抗氧化能力,减少氧化应激损伤相关,并呈现一定剂量依赖的特点。Objective:To explore the protective effect and mechanism of 919 syrup on liver function in rats with non-alcoholic fatty liver disease(NAFLD).Methods:The rat model of NAFLD was replicated by feeding high-fat diet.SD rats were divided into normal group,model group,919 syrup high-dose group,and 919 syrup low-dose group,with 8 rats in each group.The rats in the normal group were not given drugs,and the rats in the model group were given sodium chloride by gavage at a dose of 10ml/kg per day,and the rats in the high-dose group were given 919 syrup by gavage at a dose of 27g/kg per day.The rats in the low-dose group were administered 919 syrup by gavage at a daily dose of 6.75 g/kg.After 8 weeks of intervention,enzyme-linked immunosorbent assay was used to detect serum triglyceride(TG),total cholesterol(TC),alanine aminotransferase(ALT)and aspartate aminotransferase(AST),as well as the activity of superoxide dismutase(SOD)and the content of malondialdehyde(MDA)in liver tissue,the morphology of liver tissue was observed under HE staining light microscope,and the differences between the groups were compared.Results:Compared with the normal group,the liver function levels of TG,TC,ALT and AST in the model group increased,and the SOD decreased,which was significantly different(P<0.01).Compared with the model group,TG,TC,ALT,AST and MDA in the high-dose group and the low-dose group decreased,and SOD content increased,with statistical significance(P<0.05).Compared with the high and low dose groups,the levels of TG,TC and ALT were statistically significant(P<0.05).The results of HE staining showed that the hepatocytes in the model group were disorganized,swollen,and inflammatory infiltration.Both the high-dose group and the low-dose group could improve the pathological state of hepatocytes.Conclusion:919 syrup can effectively protect liver function and reduce liver cell damage in NAFLD rats,and its mechanism may be related to enhancing the level of antioxidant defense and reducing oxidative stress injury,and it shows a certa

关 键 词：919糖浆 非酒精性脂肪肝 氧化应激 肝功能 

分 类 号：R573.9[医药卫生—消化系统]