肉制品中多种食源性兴奋剂质控样品的研制及评价-以火腿肠为例  

Development and evaluation of quality control samples for multiple edible stimulants in meat products-Ham sausage as an example

作　　者：曾永福 冉洽闻 黄雪静 张海超 王敬 艾连峰 汪勇 ZENG Yongfu;RAN Qiawen;HUANG Xuejing;ZHANG Haichao;WANG jing;AI Lianfeng;WANG Yong(School of Public Health,Hebei Medical University,Shijiazhuang,050017,China;Hebei Key Laboratory of Environment and Population Health,Shijiazhuang,050017,China;China Petroleum Pipeline Bureau Engineering Company Limited Asia Pacific Branch,Langfang,,065000,China;Shijiazhuang Customs Technology Center,Shijiazhuang,050051,China;Shimadzu(China)Co.,LTD,Beijing,100020,China)

机构地区：[1]河北医科大学,公共卫生学院,石家庄050017 [2]河北省环境与人群健康重点实验室,石家庄050017 [3]中国石油管道局工程有限公司亚太分公司,廊坊065000 [4]石家庄海关技术中心,石家庄050051 [5]岛津企业管理(中国)有限公司,北京100020

出　　处：《环境化学》2022年第10期3469-3472,共4页Environmental Chemistry

基　　金：河北省技术创新引导计划项目(21475501D)资助.

摘　　要：本研究以火腿肠为基质,研制了肉制品中8种食源性兴奋剂质控样品的制备方法并对质控样品进行了分析评价.方法以β-受体激动剂、蛋白同化剂、糖皮质激素和利尿剂为目标物采用真空冷冻干燥,基质添加的方式制备质控样品,采用液相色谱-串联质谱法对质控样品中目标物进行测定.质控样品经F检验后具有良好的均匀性,经t检验后在常温(25℃)存放15d以及低温(−20℃)存放6个月具有良好的稳定性,并通过10家实验室对质控样品进行联合定值,最终确定质控样品最终定值结果:喷布特罗为(5.69±0.412)μg·kg^(−1)、克伦特罗为(2.96±0.270)μg·kg^(−1)、丙酸睾酮为(12.7±1.642)μg·kg^(−1)、睾酮为(12.6±1.633)μg·kg^(−1)、可的松为(9.85±1.145)μg·kg^(−1)、甲基泼尼松龙为(5.92±0.534)μg·kg^(−1)、氯噻嗪为(13.2±1.721)μg·kg^(−1)、托拉塞米为(17.5±2.341)μg·kg^(−1).In this study,8 kinds of food stimulant quality control samples in meat products were prepared using ham sausage as the matrix,and the quality control samples were analyzed and evaluated.The target substances ofβ-agonist,anabolic agent,glucocorticoid and diuretic were prepared by vacuum freeze-drying and matrix addition,and the target substances were determined by liquid chromatography-tandem mass spectrometry in the mass control samples.The quality control samples had good homogeneity after F-test and good stability after t-test at room temperature(25℃)for 15 days and at low temperature(−20℃)for 6 months,and through the 10 laboratories on the quality control samples for joint determination,and finally determine the final quality control sample determination results:Penbutolol to(5.69±0.412)μg·kg^(−1),clenbuterol to(2.96±0.270)μg·kg^(−1),testosterone propionate to(12.7±1.642)μg·kg^(−1),testosterone to(12.6±1.633)μg·kg^(−1),cortisone to(9.85±1.145)μg·kg^(−1),methylprednisone to(5.92±0.534)μg·kg^(−1),chlorothiazide to(13.2±1.721)μg·kg^(−1),torsemide to(17.5±2.341)μg·kg^(−1).The preparation techniques of edible stimulant quality control samples of multiple species and levels in meat products established were rigorous and reliable,and can be used for quality control and blind sample assessment of edible stimulant residue analysis.

关 键 词：食源性兴奋剂 质控样品 均匀性 稳定性 定值 肉制品 

分 类 号：TS251.7[轻工技术与工程—农产品加工及贮藏工程]