混菌发酵牛蒡子转化合成牛蒡子苷元的研究  

Study on the synthesis of arctigenin from Arctii Fructus by mixed fermentation

作　　者：孙科[1] 王宇翔 尹雷 张磊磊 齐素华 SUN Ke;WANG Yuxiang;YIN Lei;ZHANG Leilei;QI Suhua(Xuzhou Vocational College of Bioengineering,Xuzhou Biopharmaceutical Engineering Research Centre,Xuzhou 221006;Xuzhou Medical University School of Pharmacy,Xuzhou 221004)

机构地区：[1]徐州生物工程职业技术学院徐州市生物制药工程技术研究中心,徐州221006 [2]徐州医科大学药学院,徐州221004

出　　处：《中国食品添加剂》2022年第10期59-68,共10页China Food Additives

基　　金：江苏省高职院校专业带头人高端研修项目(No.2021GRFX075);徐州市科技计划项目(No.KC20051);江苏高校“青蓝工程”资助基金项目(No.JS1111)。

摘　　要：牛蒡子苷元(Arctigenin,ARG)是一种重要的广谱抗肿瘤药物。为利用微生物转化法制备牛蒡子苷元,以牛蒡子粉为底物以黑曲霉(Aspergillus niger)和里氏木霉(Trichoderma reesei)为菌种,混菌发酵转化合成牛蒡子苷元,利用高效液相色谱(High Performance Liqued Chromatography,HPLC)分析测定牛蒡子苷元。通过单因素试验对20L发酵罐进行最佳条件研究,结果表明:接种量为15%、发酵温度为29℃、搅拌转速为350r/min、通气量为1.0VVM。在单因素试验的基础上,采用Box-Behnken中心组合试验原理设计响应面方法优化工艺条件,得到最优发酵工艺条件为:接种量为14%、发酵温度为29℃、搅拌转速为335r/min、通气量为0.9VVM。在此条件下,牛蒡子苷元增加量为17.24mg/mL、牛蒡子苷转化率为98.7%。研究结果为微生物发酵转化合成牛蒡子苷元提供理论和实践基础,拓展了天然药用植物开发的路径。Arctigenin(ARG)is an important broad-spectrum anti-tumor drug.In order to prepare arctigenin by microbial transformation,arctigenin was synthesized by fermentation of mixture of Aspergillus Niger and Trichoderma Reesei with arctigenin powder as substrate,determination of arctigenin by High Performance liquid Chromatography(HPLC).The optimum conditions of 20L fermentor were studied by single factor experiment.The results showed that the inoculation amount was 15%,the fermentation temperature was 29℃,the agitation speed was 350r/min,and the aeration rate was 1.0VVM.On the basis of single factor test,the response surface method was used to optimize the process conditions by using the Box-Behnken central composite test principle,the optimal fermentation conditions were as follows:inoculation amount was 14%,fermentation temperature was 29℃,agitation speed was335r/min,aeration volume was 0.9VVM.Under these conditions,the content of Arctigenin increased to 17.24mg/mL,and the conversion rate of Arctigenin was 98.7%.The results provide theoretical and practical basis for the microbial fermentation of arctigenin,and expand the path of natural medicinal plants development.

关 键 词：混菌发酵 转化 牛蒡子 牛蒡子苷 牛蒡子苷元 

分 类 号：Q815[生物学—生物工程] R286.0[医药卫生—中药学]